| 水稻条叶枯病毒基因产物在水稻和昆虫体内的Western印迹分析 |
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| 引用本文： | 曲志才,沈大棱,徐亚南,谈家桢,RogerHULL.水稻条叶枯病毒基因产物在水稻和昆虫体内的Western印迹分析[J].遗传学报,1999(5). |
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| 作者姓名： | 曲志才 沈大棱 徐亚南 谈家桢 RogerHULL |
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| 作者单位： | 复旦大学遗传学研究所!上海，200433，复旦大学遗传学研究所!上海，200433，复旦大学遗传学研究所!上海，200433，复旦大学遗传学研究所!上海，200433 |
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| 基金项目： | 美国McKnight基金，上海市科委自然科学基金 |
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| 摘 要： | 将AStV基因组组分3编码的NS3和NC蛋白基因以及组分4编码的NCP和NSvc4蛋白基因分别克隆于大肠杆菌表达载体pGEX3X在IPTG诱导下，表达出4种融合蛋白。这些表达产物用于免疫动物产生多克隆抗血清。以制备的多克隆抗血清为探针，Western印迹分析了宿主植物和介体昆虫体内NS3、NC、NCP和NSvc4种基因表达的产物。NC蛋白的抗体可在病株和病毒粒子制备物中以及NCP抗血清仅在病株中检测到相应大小的产物，其余的均与预期值相停。
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| 关 键 词： | 病毒基因 基因表达 融合蛋白 印迹分析 |
Western Blotting of RSF Gene Products in Rice and Insects |
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| QU Zhi-Cai , SHEN Da-LENG, XU Ya-Nan , TAN Jia-Zhen , Roger HULL.Western Blotting of RSF Gene Products in Rice and Insects[J].Journal of Genetics and Genomics,1999(5). |
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| Authors: | QU Zhi-Cai SHEN Da-LENG XU Ya-Nan TAN Jia-Zhen Roger HULL |
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| Institution: | QU Zhi-Cai ; SHEN Da-LENG; XU Ya-Nan ; TAN Jia-Zhen ; Roger HULL(Institute of Genetics Fudan University Shanghai 200433)(Dept. of Virus Research John Innes Centre Norwich UK) |
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| Abstract: | The NS3 and NC protein genes encoded by RNA3 of RStV, the NCP and NSvc4protein genes encoded by RNA4 were subcloned into the E. coli expression vectorpGEX3X to express four groups of fusion protein under IPTG induction. These fusionproteins were used to immunize rabbits to raise antisera. The antisera against the Ecoli-expressed proteins were available for probing the presence of the viral geneproducts in both rice plant and insect hosts. The expected gene products can beprobed only in diseased rice plant with NCP antiserum and the cormsponding productsdetected in both plant and RStV panicle preparation with NC anhserum. The viralgene products probed by NS3 and NSvc4 anhsera were different from the expectedones in size. |
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| Keywords: | Viral gene Gene expression Fusion protein Blotting analysis |
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