| 花苜蓿小尺度空间遗传结构研究 |
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| 引用本文： | 刘霞,楚海家,陈丽,乔匿骎,闫娟.花苜蓿小尺度空间遗传结构研究[J].植物科学学报,2021,38(5):663-670. |
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| 作者姓名： | 刘霞 楚海家 陈丽 乔匿骎 闫娟 |
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| 作者单位： | 1. 西藏大学理学院, 拉萨 850000;
2. 西藏大学青藏高原生态与环境研究中心, 拉萨 850000;
3. 中国科学院武汉植物园, 武汉 430074;
4. 湖南农业大学生物科学技术学院, 长沙 410128 |
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| 基金项目： | 国家自然科学基金（31000147）；中国科学院武汉植物园人才部署专项（55Y755271G02）。 |
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| 摘 要： | 以花苜蓿（Medicago ruthenica Trautv.）为材料，在内蒙古克什克腾旗建立两个25 m×50 m的样方（山谷YF1和山坡YF2），采集该范围内的所有个体，利用8对SSR分子标记对其遗传变异特性进行分析。结果显示，克什克腾旗花苜蓿居群遗传多样性较高。两个居群个体的空间自相关分析结果表明，9 m内的个体间为非随机邻近交配，且在同距离范围内，山谷居群的个体间遗传相似性更低，推测此区域可能是历史和地理因素塑造了花苜蓿丰富的遗传多样性，两个小尺度空间格局的个体间基因流模式主要受地形影响。
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| 关 键 词： | 花苜蓿 小尺度空间遗传结构 基因流 空间自相关 |
Fine-scale genetic structure of Medicago ruthenica Trautv. |
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| Liu Xia,Chu Hai-Jia,Chen Li,Qiao Ni-Qin,Yan Juan.Fine-scale genetic structure of Medicago ruthenica Trautv.[J].Plant Science Journal,2021,38(5):663-670. |
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| Authors: | Liu Xia Chu Hai-Jia Chen Li Qiao Ni-Qin Yan Juan |
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| Institution: | 1. College of Science, Tibet University, Lhasa 850000, China;
2. Research Center for Ecology and Environment of Qinghai-Tibetan Plateau, Tibet University, Lhasa 850000, China;
3. Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China;
4. College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China |
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| Abstract: | We established two quadrats (25 m×50 m) in a valley (YF1) and on a hillside (YF2) in Keshiketeng Banner, Inner Mongolia. We sampled all Medicago ruthenica Trautv. individuals in the quadrats by fine-scale methods and characterized their genetic variation using eight pairs of simple sequence repeat (SSR) primers. We found that M. ruthenica had a high level of genetic diversity. Based on spatial autocorrelation, the individuals showed non-random adjacent mating at an interval of 9 m. At the same interval, we found lower genetic similarity in the YF1 population than in the YF2 population. Thus, we inferred that the rich genetic diversity of M. ruthenica might be shaped by historical and geographic factors in this area, with topography impacting gene flow patterns between the two fine-scale populations. |
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| Keywords: | Medicago ruthenica Fine-scale spatial genetic structure Gene flow Spatial autocorrelation |
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