影响辣椒离体再生芽伸长因素研究(英文)

以辣椒子叶为外植体,比较不同浓度BA和IAA激素组合对辣椒再生芽诱导的差异,利用筛选出的高效芽诱导培养基为基础,研究了赤霉素、芽诱导时间、培养基有机成分、不同激素组合和品种等因素对辣椒不定芽伸长的影响。结果表明:不同基因型辣椒子叶再生能力不同,BA3.0mg·L^-1+IAA0.5mg·L^-1的激素配比对不定芽诱导频率最高;不定芽的伸长百分率随着GA₃浓度的增加而增加,GA₃的适合浓度为1.0～2.0mg·L^-1;不定芽诱导时间对不定芽的伸长有一定的影响,诱导21d的不定芽,其伸长频率明显高于诱导14d的不定芽;B5有机成分在辣椒不定芽的伸长中效果优于MS有机成分;激素组合对不定芽伸长有一定的影响,Zeatin+GA₃激素组合对伸长效果最好,BA+IAA+GA₃伸长效果较好,BA+PAA(苯乙酸,phenylaceticacid)+GA₃伸长效果次之;不同品种辣椒不定芽的伸长能力有一定差异,楚风和苏椒五号再生芽伸长能力最佳。与IAA和NAA相比,IBA对再生芽生根效果较好。

Factors Affecting in vitro Shoot Elongation of Pepper

A detailed study was carried out to investigate key factors affecting shoot elongation of pepper.Using the cotyledons of pepper as explants,the optimal phytohormone combination of BA+IAA for bud induction was screened out to investigate the effect of GA₃,bud-inducting time,organic constituents in media,different phytohormone combinations and cultivars on shoot elongation.The results were as follows:Seven cultivars showed ability differences for bud induction,and BA 3.0 mg·L^-1+IAA 0.5 mg·L^-1 could give the highest response.The shoot elongation frequency increased with GA₃ concentration,with the optimal concentration of 1.0-2.0 mg·L^-1.The buds subjected to 21-day induction showed higher elongation efficiency than that of 14-day induction.B5 vitamins were more efficient than MS vitamins for shoot elongation.The phytohormone combination of Zeatin+GA₃ exhibited higher elongation efficiency than combinations of BA+IAA+GA₃ or BA+PAA+GA₃.Elongation ability varied with pepper cultivars,with Chufeng and Sujiao No.5 having the best elongation ability.Compared with IAA and NAA,IBA gave better rooting effect.