芒草中总黄酮的提取工艺研究及其挥发油成分的鉴定

以黄酮提取率为指标,采用超声辅助法研究了芒草中黄酮类化合物的提取工艺。最佳提取条件为:乙醇浓度70%、料液比1∶30、提取温度70℃、提取时间40 min、提取级数3次,在此条件下测得南荻中总黄酮含量为7.24 mg/g,并在此条件下测定了芒草10个品系中总黄酮含量为4.16~7.84 mg/g,且在不同品系间的差异均达到极显著水平。采用气质联用技术鉴定奇岗六倍体、‘GMG121’、芒杂中的挥发油成分,从中共鉴定出28种成分。本研究对芒属植物的遗传育种以及开发和综合利用具有重要意义。

Optimal Total Flavonoids Extraction and Identification of Volatile Oil Components in Miscanthus sinensis

An ultrasonic assisted method was used to study the optimum extraction of flavonoids in Miscanthus. The determined optimal extraction conditions of flavonoids were as follows:70% ethanol concentration, 1:30 ratio of raw material to liquid, 70℃ extraction temperature, 40 min extraction time, and three extract series, respectively. Under these conditions, total flavonoid content of M.lutarioriparius was 7.24 mg/g. The experimental yields of flavonoids from various kinds of M.sinensis were significantly different, ranging from 4.16 mg/g to 7.84 mg/g. The volatile oil components in Miscanthus x giganteus (hexaploid),‘GMG121’, and M.sinensis hybrid were determined by GC/MS, with 28 different components identified. This study provides useful information on genetic breeding, which has great significance for the development and utilization of M.sinensis.