阴地蕨属药用植物matK基因编码区全序列测定及编码产物研究

以阴地蕨属(Botrychium)5种药用植物的matK基因为对象,分析matK基因编码区全序列和其编码产物MATK蛋白的氨基酸序列特征,并比较他们在用于阴地蕨属药用植物系统发育关系研究中的差异。结果显示,阴地蕨属5种植物matK基因全长为1500~1503 bp,共有153个变异位点,其编码产物均为不稳定的亲水性蛋白,无跨膜结构,二级结构以α-螺旋和无规则卷曲为主。系统发育分析结果表明,基于matK基因序列的系统发育分析更适合于阴地蕨属种间亲缘关系的鉴定,说明matK基因在阴地蕨属植物的鉴定中具有一定的应用价值。

Study on full-length coding sequence of matK gene and its coding protein from medicinal Botrychium

The matK gene plays an important role as the only group Ⅱ intros maturase in the chloroplast. We carried out bioinformatics analysis of the matK gene from five species of Botrychium. We then discussed the differences in matK gene sequences and MATK protein amino acid sequences in phylogenetic development of Botrychium. Results showed that the full length of the matK gene of the five species ranged from 1500-1503 bp, with 153 variation sites. The encoding products were all unstable hydrophilic proteins without a transmembrane structure, and the secondary structures were mainly alpha helices and random coils. Phylogenetic results showed that the phylogenetic tree constructed from the matK gene sequence was suitable for the identification of species of Botrychium. This study enriches current molecular information on matK in Botrychium and establishes the application value of matK in the identification of Botrychium.