The contribution of endophytic bacteria to Albizia lebbeck-mediated phytoremediation of tannery effluent contaminated soil |
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| Authors: | Muthu Manikandan Vijayaraghavan Kannan Ordetta Hannah Mendoza Mahalingam Kanimozhi Lejla Pašić |
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| Institution: | 1. Department of Bioresource and Food Science, Konkuk University, Seoul, Korea;2. Center for Advanced Studies in Botany, University of Madras Guindy Campus, Chennai, India;3. Department of Botany, Stella Maris College, Chennai, India;4. Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia |
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| Abstract: | Toxicity of chromium often impairs the remediation capacity of plants used in phytoremediation of polluted soils. In this study, we have identified Albizia lebbeck as a prospective chromium hyperaccumulator and examined cultivable diversity of endophytes present in chromium-treated and control saplings. High numbers (22–100%) of endophytic bacteria, isolated from root, stem, and leaf tissues, could tolerate elevated (1–3 mM) concentrations of K2CrO7. 16S rRNA gene sequence-based phylogenetic analysis showed that the 118 isolates obtained comprised of 17 operational taxonomic units affiliated with the proteobacterial genera Rhizobium (18%), Marinomonas (1%), Pseudomonas (16%), and Xanthomonas (7%) but also with members of Firmicutes genera, such as Bacillus (35%) and Salinococcus (3%). The novel isolates belonging to Salinococcus and Bacillus could tolerate high K2CrO7 concentrations (3 mM) and also showed elevated activity of chromate reductase. In addition, majority (%) of the endophytic isolates also showed production of indole-3-acetic acid. Taken together, our results indicate that the innate endophytic bacterial community assists plants in reducing heavy metal toxicity. |
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| Keywords: | chromium remediation phytoremediation endophytic bacteria chromate reductase tannery effluent |
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