利用F-MSAP分析菜心表观遗传多样性

菜心杂交除了引起DNA序列变化,还可能引起不依赖于DNA序列的表观遗传变化,为揭示菜心表观遗传多样性形成机理,该文利用F-MSAP检测49份菜心的DNA甲基化水平和模式变化。结果表明:(1)F-MSAP检测效率较高,菜心DNA甲基化多态性较高,杂交可以提高DNA甲基化多态性。(2)菜心表观遗传多样性较低,均质化严重,大部分遗传变异来源于种内,自交增加自交系的表观遗传差异,杂交增加杂种的表观遗传差异。(3)49份菜心的DNA甲基化水平较高,以全甲基化模式为主,自交降低DNA甲基化水平,杂交通过DNA甲基化模式变化增加自交系杂种的DNA甲基化水平。(4)49份菜心分成五类,聚类分析和主成分分析结果基本一致,杂种倾向于按照母本亲缘关系分类。该研究利用F-MSAP检测菜心表观遗传多样性,提高了菜心的鉴定效率和准确性,为进一步开展杂交育种提供了理论基础和技术支持。

Epigenetic diversity of Chinese flowering cabbage revealed by F-MSAP

In addition to DNA sequence changes, the hybridization Chinese flowering cabbage may also cause epigenetic changes that are independent of DNA sequence. In order to reveal the formation mechanism of epigenetic diversity in Chinese flowering cabbage, the changes on the level and pattern of DNA methylation of 49 Chinese flowering cabbages were tested by F-MSAP. The results were as follows:(1)The detection efficiency of F-MSAP was high, the DNA methylation polymorphism of Chinese flowering cabbage was high, and hybridization could improve the DNA methylation polymorphism.(2)The epigenetic diversity of cabbages was low, the homogenization was serious, and most of the genetic variation was originated from within the species. Selfing increased the epigenetic differences between inbred parents, hybridization increased the epigenetic difference of hybrids.(3)The DNA methylation level of 49 Chinese flowering cabbages was relatively high, the pattern was mainly full methylation. Selfing decreased the DNA methylation level, and hybridization increased the DNA methylation level of inbred hybrids through the change of DNA methylation pattern.(4)The 49 Chinese flowering cabbages were divided into five categories. The results of cluster analysis and principal component analysis were basically consistent. Hybrids tended to be classified according to female parent genetic relationship. This study improves the identification efficiency and accuracy through the analysis of epigenetic diversity of Chinese flowering cabbage, and provides theoretical basis and technical support for further cross-breeding.