采用高效液相色谱技术分析烟草体内的维生素B6化合物

维生素B6 (VB6)是一类化合物的总称.近年来研究发现VB6在植物体内发挥抗逆作用.烟草作为模式植物其体内VB6的存在形态还未见报道.本研究采用高效液相色谱结合荧光检测技术对烟草体内VB6的存在形态进行了分析.结果表明:土壤栽培烟草叶、茎和根中VB6的含量依次为2.9、1.7、3.0 μg/g鲜重;组培烟草叶片的VB...

Analysis of vitamin B_6 vitamers in tobacco plants by high performance liquid chromatography

Vitamin B_6(VB_6)is the general term for a kind of chemical compounds .VB_6 exists in several forms,and has been linked to stress responses in plants. Until now no reports about the distribution of B_6 vitamers in tobacco plants have been observed. In our experiment,the determination of VB_6 vitamers in tobacco plants was described by using HPLC with fluorescence detector. The results indicated that,the contents of VB_6 in leaves,tender stem and roots were 2.9,1.7 and 3.0 μg/g fresh weight,respectively. Leaves of tobacco plants grown on MS basal media exhibited a high content of 3.9 μg/g fresh weight. The constituent ratio of B_6 vitamers were as follows: pyridoxamine 5′-phosphate(PMP) 7%,pyridoxamine(PM) 14%,pyridoxal 5′-phosphate(PLP) 19%,pyridoxal(PL) 29% and pyridoxine(PN) 30%. During the determination period of three weeks,the contents of PLP and PL decreased,and that of PN increased. The amount of VB_6 was relatively constant. Our results would be favorable for further study on the metabolic mechanism and special physiological mechanism in tobacco plants.