| 水稻花药愈伤组织诱导的多因子正交试验研究 |
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| 引用本文： | 王辉,肖小君,齐泽民,黄作喜,王芳.水稻花药愈伤组织诱导的多因子正交试验研究[J].广西植物,2011,31(4):545-549. |
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| 作者姓名： | 王辉 肖小君 齐泽民 黄作喜 王芳 |
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| 作者单位： | 内江师范学院生命科学学院,四川省高等学校特色农业资源研究与利用重点实验室,四川内江641112 |
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| 摘 要： | 以密阳46和培矮64/8006杂交F1代的花药为材料,研究两个材料花粉发育时期与叶枕距大小的相关性.结果表明,密阳46在叶枕距5～7 cm时,小孢子大部分处于单核靠边期；培矮64/8006杂交F1代稍迟,叶枕距为7～9 cm时,大部分小孢子才进入单核靠边期.同时通过L9(34)正交试验,研究了培养基类型、激素配比、蔗糖...
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| 关 键 词： | 水稻 花药 愈伤组织诱导 正交试验 |
Studies on anther callus induction of rice by orthogonal test |
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| WANG Hui,XIAO Xiao-Jun,QI Ze-Min,HUANG Zuo-Xi,WANG Fang.Studies on anther callus induction of rice by orthogonal test[J].Guihaia,2011,31(4):545-549. |
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| Authors: | WANG Hui XIAO Xiao-Jun QI Ze-Min HUANG Zuo-Xi WANG Fang |
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| Institution: | WANG Hui,XIAO Xiao-Jun,QI Ze-Min,HUANG Zuo-Xi,WANG Fang(School of Life Science,Neijiang Normal University,Key Laboratory of Colleges and Universities in Sichuan for Research and Utilization of Distinctive Agricultural Undertakings,Neijiang 641112,China) |
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| Abstract: | Using the anther of Milyang 46 and the F1 hybrids of Pei'ai 64/8006,the correlation of pollen development with the distance of the pulvinus was studied.The results showed that most of the microspores were in the late uninucleate stage when the distance of the pulvinus was 5-7 cm for Milyang 46;while it was 7-9 cm for the F1 hybrids of Pei'ai 64/8006.Besides,the effects on anther callus induction rate of the types of medium,the ration of plant hormones,the concentration of the sucrose and 2,4-D were investig... |
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| Keywords: | rice anther callus induction orthogonal test |
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