应用RAPD和ISSR标记对24份花生栽培种材料进行遗传多样性分析

利用RAPD引物和ISSR引物分析我国24份花生栽培种材料的遗传多样性.结果表明:所选的RAPD引物和ISSR引物中分别有13条引物和10条引物扩增出了清晰并可重复的条带,共扩增出123条带和87条带,平均每条引物扩增出9.5条带和8.7条带,其中多态性带分别占条带总数的47.15％和57.47％,平均每条引物扩增出4...

Genetic diversity in 24 peanut cultivars as revealed by RAPD/ISSR profiling

Genetic diversity in 24 accessions of cultivated peanut materials from China was evaluated based on RAPD/ISSR profiling. Of the RAPD primers and ISSR primers tested,13 and 10 primers produced a total of 123 and 87 repeatable,clear and readable bands,among which 47.15% and 57.47% were polymorphic,respectively. On average,a primer resulted in 9.5 and 8.7 bands,of which 4.7 and 5.7 were polymorphic. A dendrogram was constructed using UPGMA algorithm based on Nei and Li’s similarity coefficient,which divided the 24 peanut materials into 4 groups. Silihong,Sanyou 523,Jihua 2,Huayu 16 and Yueyou 7 were in one group. Huyu 20 and Zhonghua 8 clustered together. Heihuasheng formed a separated group,and the rest peanut materials tested fell in a group. RAPD and ISSR are useful for the genetic diversity studies of the cultivated peanut,and have potential in characterization of peanut germplasm and gene mapping.