兰州百合组培鳞茎发育研究

该研究以兰州百合商品种球鳞片为外植体材料，通过组织培养诱导丛生芽萌发及高频增殖，再以丛生芽为材料诱导其发育形成小鳞茎，调节培养基对小鳞茎进行膨大发育培养，最终形成促进兰州百合组培鳞茎膨大发育的“三步”组培培养技术路线；对发育过程中形成的丛生芽、小鳞茎及膨大鳞茎进行淀粉含量测定与生长特征参数统计，分析各步培养对鳞茎形成发育过程中淀粉含量与形态变化的影响。结果表明：所建立的“三步”培养方案培育出的组培鳞茎直径、重量与鳞片数分别为1.66 cm、2.48 g和26.33片，有效地促进了鳞茎的膨大，并能诱导鳞茎主茎杆的形成发育；在培养进程中其淀粉含量呈现逐步增加的趋势，这表明与鳞茎膨大发育正相关，同时鳞茎大小、重量及鳞片数三者也表现为正相关性；当鳞茎所含鳞片数在26片以上时，其生长点易发育形成主茎杆。该文研究了兰州百合组培鳞茎的形成与膨大发育技术，所研发的“三步”培养组成的鳞茎膨大发育组培技术有效地促进了鳞茎的膨大发育，而膨大发育的鳞茎能有效地缩短田间生长周期，从时间上提高百合生产量，同时为实现兰州百合膨大的鳞茎种球规模化生产提供技术支撑。

Bulblet formation and development of Lanzhou lily (Lilium davidii var. unicolor)by tissue culture

Bulblet formation and development of Lanzhou lily(Lilium davidii var. unicolor)by tissue culture could provide technical solutions to realize the mass production of Lanzhou lily bulblet, which needs three steps to achieve, including multiple shoots proliferation, induction of bulblets and enhancement growth of bulblets. The starch content and the characteristic parameters of bulblet were detected at different culture stages. This study acquired an advanced technique that could effectively promote the bulblet formation and development, and induce the growth of main stem. The results demonstrated that the bulblet diameter, weight and number of scales were up to 1.66 cm, 2.48 g and 26.33 pieces, respectively. The starch content showed a gradual increasing trend according to the culture process, which indicated that the starch content was positively correlated to the development of bulblet. In addition, the bulblet size, weight and number of scales showed a positive correlation. The growing point of bulblet was easy to form the main stem when the number of scales reached to 26 or more. In this paper, the invented three-step tissue culture technology effectively promotes bulblet enhancement development, and enlargement of the bulblet can effectively shorten the field growth cycle, improve lily production on time, and also, it can provide technical reference for achievement of mass production of enhancement bulblet.