圣剑'洋桔梗植株高频再生体系建立及卡那霉素敏感性测定

洋桔梗是国际上十分流行的盆花和切花种类。以洋桔梗‘圣剑’无菌苗叶片为外植体,研究了6-BA与NAA不同浓度组合对其不定芽再生的影响,并分别比较了不同浓度IBA和NAA诱导其生根的效果,测定了该品种在不定芽再生时对卡那霉素(Km)的敏感性。结果表明:MS+0.5 mg·L-16-BA+0.01 mg·L-1NAA为不定芽再生最适培养基,不定芽再生率达91%;1/2 MS+0.2 mg·L-1IBA为不定根再生的最适培养基,生根率达89%;抑制叶片不定芽再生的Km最低浓度为25 mg·L-1。建立了‘圣剑’洋桔梗植株高频再生体系,并确定了其对卡那霉素的敏感性,为该品种的基因工程研究奠定了基础。

Establishment of high frequency regeneration system for Eustoma grandiflorum cv. Excalibur Blue Picotee and determination of kanamycin sensitivity

Eustoma grandiflorum is a popular cut flower and pot flower in the world. In this study,using leaves of sterile plantlets of E. grandiflorum cv. Excalibur Blue Picotee as explants,the effects of different concentration combinations of 6-BA and NAA on adventitious bud regeneration were investigated. The effects of different concentrations of IBA and NAA on rooting were studied,respectively. The sensitivity to kanamycin upon the regeneration of adventitious buds was determined. The results showed that the medium with MS+0.5 mg·L^-1 6-BA+0.01 mg·L^-1 NAA was the optimum medium for adventitious bud regeneration,with 91% regeneration rate. Meanwhile,the medium with 1/2MS+0.2 mg·L^-1 IBA was the optimum medium for rooting,and the rooting rate reached 89%. Besides,the minimum concentration of Km inhibiting the regeneration of adventitious buds was 25 mg·L^-1. This study laid an important foundation for the research of genetic engineering on this cultivar of E. grandiflorum.