| 黄芪原生质体分离技术 |
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| 引用本文： | 刘,强,张宗申,丛丽娜,章凌雪.黄芪原生质体分离技术[J].广西植物,2008,28(3):411-413. |
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| 作者姓名： | 刘 强 张宗申 丛丽娜 章凌雪 |
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| 作者单位： | 大连工业大学,生物与食品工程学院,辽宁,大连,116034 |
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| 摘 要： | 以黄芪叶片和愈伤组织为材料,对黄芪原生质体的制备分离技术进行了研究。结果表明:采用黄芪叶片制备原生质体远远优于黄芪愈伤组织,能够获得大量高活力的原生质体;采用2%纤维素酶+0.5%半纤维素酶+0.5%果胶酶的混合酶水解12h就能达到较好的分离效果,获得高质量的黄芪原生质体,然后进行原生质体的培养。
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| 关 键 词： | 黄芪 原生质体 愈伤组织 组织培养 |
| 文章编号： | 1000-3142(2008)03-0411-03 |
| 修稿时间： | 2006年12月14 |
Isolation of protoplast from the Astragalus membranaceus |
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| LIU Qiang,ZHANG Zong-Shen,CONG Li-N,ZHANG Ling-Xue.Isolation of protoplast from the Astragalus membranaceus[J].Guihaia,2008,28(3):411-413. |
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| Authors: | LIU Qiang ZHANG Zong-Shen CONG Li-N ZHANG Ling-Xue |
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| Institution: | College of Bio & Food Technology, Dalian Institute of Light Industry, Dalian 116034, China |
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| Abstract: | Leaves and callus of the Astragalus membranaceus were used to examine the conditions for protoplast preparation and culture of the A.membranaceus.The results showed that the leaf segment of the A.membransceus could release massive and vigorous protoplasts.Compared with the callus of the species,the protoplasts extracted from the leaf segment are more efficient in the cell fusion and tissue culture.The optimized treatment is 2% cellulase,0.5% hemicellulase,and 0.5% pectinase for 12 h. |
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| Keywords: | Astragalus membranaceus protoplast callus tissue culture |
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