八角莲组织培养研究

以八角莲种子为外植体,MS为基本培养基,通过不同的激素种类和浓度配比,对八角莲进行组织培养研究。结果表明:种子在MS+BA1.0mg/L+IBA0.5mg/L+GA34.0mg/L培养基上容易萌芽,发芽率为72.4 %;培养基MS+BA10.0mg/L+GA30.5 mg/L可诱导种子幼苗形成丛生芽;继代繁殖在MS+BA(8.0～10 .0)mg/L+GA32 .0mg/L与低浓度BA或无BA的培养基上进行循环培养效果较好;MS+NAA1.0 mg/L+AC0.2g/L适宜诱导生根获得再生植株,生根率100%。带叶叶柄在MS+BA1.0mg/L+2-ip(0.5～1.0) mg/L+NAA0.02 mg/L培养基上可诱导愈伤及根,直接形成再生植株。生根苗移栽成活率90 %。

Study on tissue culture of Dysosma versipellis

The seeds of Dysosma versipellis were used as explants for tissue culture in different culture media. The results obtained from the present study were as follows:the seed germination rate could reach 72.4% on MS+BA1.0 mg/L+IBA0.5 mg/L+GA34.0 mg/L;the medium which was good for the cluster buds induction in seed buds was MS+BA10.0 mg/L+GA30.5 mg/L;the medium of proliferation should be MS+BA(8.0～10.0) mg/L+GA32.0 mg/L with low concentration of BA or no BA for circulation culture;the ideal medium for root formation was MS+NAA1.0 mg/L+AC0.2 mg/L,and the rooting rate could reach 100%;tender leaves with stalk could be induced for callus and roots in MS+BA1.0 mg/L+2-ip(0.5～1.0) mg/L+NAA0.02 mg/L. The survival rate of the seedlings was about 90%.