| 杉木第四号染色体特异性RAPD片段的获得 |
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| 引用本文： | 李湘阳,周坚.杉木第四号染色体特异性RAPD片段的获得[J].广西植物,2004,24(5):418-421,425. |
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| 作者姓名： | 李湘阳 周坚 |
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| 作者单位： | 南京林业大学森林资源与环境学院,江苏,南京,210037;中国林科院热带林业研究所,广东,广州,510520;南京林业大学森林资源与环境学院,江苏,南京,210037 |
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| 基金项目： | 国家自然科学基金资助项目(39970626) |
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| 摘 要： | 分别分离杉木(Cunninghamia lanceolata(Lamb.)Hook)同一细胞中的第四号具随体染色体及剩余20条非随体染色体,进行DOP-PCR扩增,分别以随体染色体及非随体染色体的DOP-PCR产物为模板,用成对随机引物进行RAPD分析。用引物对OPB07+OPB10进行扩增,在500-250 bp之间,随体染色体有4条特异扩增带;用引物对OPB07+OPB18在900 bp左右获得1条随体染色体特异带;用引物对OPD07+OPD05在250 bp左右得到随体染色体1条特异扩增带。
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| 关 键 词： | 杉木 染色体 DOP-PCR RAPD |
| 文章编号： | 1000-3142(2004)05-0418-04 |
RAPD special fragments of satellite chromosomes from Cunninghamia lanceolata |
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| LI Xiang-yang,ZHOU Jian.RAPD special fragments of satellite chromosomes from Cunninghamia lanceolata[J].Guihaia,2004,24(5):418-421,425. |
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| Authors: | LI Xiang-yang ZHOU Jian |
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| Abstract: | A pair of satellite chromosomes and 20 other non-satellite chromosomes in one cell from Cunning hamia lanceolata were isolated. The two kinds of chromosomes were amplified by DOP-PCR. And the DOP-PCR products were analyzed by RAPD-PCR using pairwtse combinations of primers. There were four specific fragments between 500-250 bp which belong to satellite chromosomes in the RAPD profile with pairwise primers OPB07 and OPB10. Another specific about 900 bp fragment of satellite chromosomes was obtained in the amplified products with pairwise primers OPB07 and OPB18. And a specific about 250 bp fragment also belonged to satellite chromosomes, which was get in the RAPD profile using pairwise primers OPD07 and OPD05. |
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| Keywords: | Cunninghamia lanceolata Chromosome DOP-PCR RAPD |
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