褐纹报春苣苔组织培养与快速繁殖

褐纹报春苣苔(Primulina glandaceistriata)是一种极具观赏价值的喀斯特地区野生花卉,目前尚未有褐纹报春苣苔组培快繁的研究报道。该研究以褐纹报春苣苔的叶片为外植体,通过两种途径建立其组培快繁体系。结果表明:适宜的不定芽诱导培养基为MS+6-BA 2.0 mg·L~(-1)+NAA 0.10 mg·L~(-1),适宜的不定芽增殖培养基为MS+ZT 2.0 mg·L~(-1)+NAA 0.10 mg·L~(-1)+活性炭0.05 g·L~(-1),增殖系数为11.09;适宜的愈伤组织诱导培养基为MS+TDZ 2.0 mg·L~(-1)+NAA 0.10 mg·L~(-1),适宜的愈伤组织分化培养基为MS+ZT 1.0 mg·L~(-1)+NAA 0.10mg·L~(-1),分化系数为12.46;适宜的生根培养基为1/2MS+IBA 0.1 mg·L~(-1)+活性炭0.05 g·L~(-1)或1/2MS+IBA0.5 mg·L~(-1)+活性炭0.05 g·L~(-1),生根率为100%。该研究结果成功建立了褐纹报春苣苔的组培快繁体系,为今后褐纹报春苣苔的种苗繁殖和遗传转化提供了技术支持。

Tissue culture and rapid propagation of Primulina glandaceistriata

Primulina glandaceistriata is a highly ornamental value of the wild flowers in karst area, yet there is no research on the tissue culture of P. glandaceistriata. We took the leaves of P. glandaceistriata as material to study tissue culture and rapid propagation by two methods. The results showed that the optimum medium for adventitious bud regeneration was MS+6-BA 2.0 mg·L^-1+NAA 0.10 mg·L^-1, the optimum medium for multiplication culture was MS+ZT 2.0 mg·L^-1+NAA 0.10 mg·L^-1+ active carbon 0.05 g·L^-1 and the multiplication coefficient was 11.09, the optimum culture medium which induced the callus was MS+TDZ 2.0 mg·L^-1+NAA 0.10 mg·L^-1, the best differentiation medium was MS+ZT 1.0 mg·L^-1+NAA 0.10 mg·L^-1 and the differentiation coefficient was 12.46, the optimum medium for rooting culture was 1/2MS+IBA0.1 mg·L^-1+ active carbon 0.05 g·L^-1 and 1/2MS+IBA0.5 mg·L^-1+ active carbon 0.05 g·L^-1, the rooting rate was 100%. In this study, tissue culture and rapid propagation system of P. glandaceistriata was successfully developed to provide a theoretical basis and technical support for future research on genetic transformation.