基于叶片生理指标的小麦芽期耐盐性评价

土壤盐渍化严重影响小麦生产,提高小麦耐盐性是应对土壤盐渍化的主要生物途径之一.小麦芽期亦是对盐分较为敏感的时期,小麦芽期耐盐性的强弱对盐碱地小麦种植至关重要.为探讨利用叶片生理指标进行小麦芽期耐盐性评价的可行性,该文以沧麦6005及其73个叠氮化钠诱变家系为试验材料,在超纯水和40％人工海水条件下,对芽期叶片中脯氨酸、...

Comprehensive evaluation of salt tolerance in wheat based on physiological indexes of leaves at germination stage

Soil salinization seriously impacts wheat(Triticum aestivum)production. Improving salinity tolerance is one of the main biological approaches responding to the problem. The germination stage is also sensitive to salt, and the salt tolerance at the germination stage is very important for wheat planting in saline-alkali land. In order to explore the feasibility of using physiological indexes of leaves to evaluate salt tolerance of wheat at germination stage, CM6005 and its 73 sodium azide mutagenesis families were used as experimental materials. The contents of proline, soluble sugar, total protein and soluble protein, as well as the activities of peroxidase(POD), catalase(CAT)and superoxide dismutase(SOD)in the leaves were measured under the conditions of ultra-pure water and 40% artificial sea water, and principal component analysis and cluster analysis were used to evaluate the salt tolerance of the wheats. The results were as follows:(1)Under 40% artificial sea water salt stress, the contents of proline and soluble sugar increased, while those of total protein and soluble protein decreased. The activity of POD increased, however, the activities of CAT and SOD decreased.(2)In principal component analysis, the first and the fourth principal components were two factors of the response of salt-tolerant enzyme system, and the second and third principal components were the main two factors of osmotic adjustment of salt tolerance in wheat leaves at the stage of germination.(3)In the evaluation of salt tolerance, the salt-tolerant grade of CM6005 was 2(salt tolerance), and that of the lines was 1(high salt tolerance), which were SAM1, SAM49 and SAM59. All the above results indicate that the activities of POD, CAT and SOD as well as the contents of proline and soluble sugar can be used as physiological indexes to evaluate the salt tolerance of wheat at germination stage. These results can be used as a reference for the evaluation of salt tolerance in wheat based on physiological indexes of leaves at the germination stage.