| 拟南芥NPR1基因的克隆与表达载体的构建 |
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| 引用本文： | 秦新民,李文兰,张丽珍,李惠敏,覃屏生.拟南芥NPR1基因的克隆与表达载体的构建[J].广西植物,2005,25(1):58-61,65. |
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| 作者姓名： | 秦新民 李文兰 张丽珍 李惠敏 覃屏生 |
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| 作者单位： | 广西师范大学生命科学学院,广西桂林,541004 |
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| 基金项目： | 广西科技攻关项目(桂科攻0228019 2) |
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| 摘 要： | NPR1基因为植物抗病基因表达和系统获得性抗性中的一个关键基因。该文以DNA PCR扩增的方法,从拟南芥基因组DNA中克隆出NPR1基因,通过序列分析,所克隆的 NPR1 基因与报道的基因序列完全一致。将其构建成植物表达载体,为今后植物抗病基因工程的开展奠定了基础。
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| 关 键 词： | 拟南芥 NPR1 克隆 基因序列分析 系统获得性抗性 |
| 文章编号： | 1000-3142(2005)01-0058-04 |
Cloning and expression vector construction of Arabidopsis NPR1 gene |
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| QIN Xin-min,LI Wen-lan,ZHANG Li-zhen,LI Hui-min,QIN Ping-sheng.Cloning and expression vector construction of Arabidopsis NPR1 gene[J].Guihaia,2005,25(1):58-61,65. |
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| Authors: | QIN Xin-min LI Wen-lan ZHANG Li-zhen LI Hui-min QIN Ping-sheng |
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| Abstract: | The Arabidopsis NPR1 gene plays an important role in activating various plant defense responses,including expression of the pathogenesis-related genes and systemic acquired resistance. The NPR1 gene was amplified from Arabidopsis thaliana genome DNA by DNA-PCR method. The DNA sequenced analysis showed that the sequence of amplified NPR1 gene was the same as the published sequence. Therefore, the plant expression vector pCaMVNPR containing NPR1 gene was constructed. The experiment will provide avenues for NPR1 gene application in plant disease resistant genetic engineering. |
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| Keywords: | Arabidopsis thaliana nonexpresser of PR genes cloning gene sequence analysis systemic acquired resistance |
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