ISSR鉴定亲缘关系非常近的芒果栽培品种

用ISSR技术鉴定7个吕宋芒品种(系)和柳州吕宋芒。从30个引物中筛选出6个多态性好的ISSR引物建立DNA指纹图谱用于区分吕宋芒品种(系)。分析DNA指纹图谱,发现这6个引物中每个引物都能区分吕宋系列品种(系),表明ISSR-PCR技术对芒果品种(系)的鉴定非常有效,能区分亲缘关系很近的品种(系)。基于69条多态性条带的聚类分析结果,发现吕宋芒和其它供试的7个品种(系)同源性低,而这7个品种(系):高州吕宋芒、湛江吕宋芒、田阳香芒、金钱芒、柳州吕宋芒、粤西一号、攀西红吕宋同源性较高,可归为一类。

Identification of closely related mango cultivars by ISSR

Seven carabao mango cultivars or lines and Liuzhou lusong mango(Mangifera indica Linn)were examined with ISSR primers.Of the 30 primers screened,6 primers gave reproducible,polymorphic DNA amplification patterns,and were selected to construct a DNA fingerprinting map to distinguished carabao mango cultivars or lines.According to the banding patterns,all cultivars tested in this study were distinguished from each other by every one of 6 selected primers and showed ample genetic diversity,indicating that ISSR-PCR was an effective method for cultivar identification of mango cultivars and lines.Based on UPGMA analysis of 69 selected bands,the carabao showed the lowest similarity to all other cultivars while Gaozhou carabao,Zhanjiang carabao,Tianyang xiangmang,Jinqianmang,Liuzhou lusong,Yueximang No.1 and Panxi red carabao could be clustered into one group.