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高效液相色谱法测定罗汉果根中两个降三萜配糖体
引用本文:卢凤来,卢艺芳,陈换莹,刘金磊,李典鹏.高效液相色谱法测定罗汉果根中两个降三萜配糖体[J].广西植物,2010,30(6):891-894.
作者姓名:卢凤来  卢艺芳  陈换莹  刘金磊  李典鹏
作者单位:1. 广西壮族自治区 中国科学院 广西植物研究所, 广西 桂林 541006; 2.广西师范大学, 广西 桂林 541004
基金项目:中科院"西部之光"人才培养计划专项,药用资源化学与药物分子工程教育部重点实验室资助项目,广西植物研究所基本业务费项目
摘    要:建立了高效液相色谱测定罗汉果根中两个结构新颖的降三萜糖苷,罗汉果酸苷乙Ⅱ(Siraitic acidⅡB)和罗汉果酸苷甲Ⅱ(Siraitic acidⅡA)含量的方法。色谱条件:色谱柱:ZORBAX SB-C18柱(4.6mm×150mm,5μm);柱温:25℃;流动相:乙腈-水(梯度洗脱:0→4min:35%乙腈;4→16min:35%→45%乙腈);流速:0.5mL/min;检测波长:230nm;进样量:10μL。结果表明,罗汉果酸苷乙Ⅱ在0.05~0.3mg/mL,罗汉果酸苷甲Ⅱ在0.05~0.25mg/mL之间线性关系良好。该方法稳定,精密度、重现性好,且样品处理简单,提取与检测时间短。

关 键 词:罗汉果酸苷乙Ⅱ  罗汉果酸苷甲Ⅱ  HPLC  测定

Determination of two nor- triterpenoid glycosides in the roots of Siraitia grosvenorii by HPLC method
LU Feng-Lai,LU Yi-Fang,CHEN Huan-Ying,LIU Jin-Lei,LI Dian-Peng.Determination of two nor- triterpenoid glycosides in the roots of Siraitia grosvenorii by HPLC method[J].Guihaia,2010,30(6):891-894.
Authors:LU Feng-Lai  LU Yi-Fang  CHEN Huan-Ying  LIU Jin-Lei  LI Dian-Peng
Institution:1. Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China; 2.Gangxi Normal University, Guilin 541004, China
Abstract:A HPLC method was developed for determining the contents of Siraitic acid ⅡB and Siraitic acid ⅡA,which were novel 29-norcucurbitane triterepene glycosides in the roots of Siraitia grosvenorii. The chromatographic analysis was carried out on a ZORBAX SB-C18 column(4.6 mm×150 mm,5 μm),and the mobile phase was acetonitrile(A)-water(gradient elution:0→4 min:0→35%A,4→16 min:35%→ 45%A). The flow rate was maintained at 0.5 mL/min. The detection wavelength was set at 230 nm,and the column temperature was controlled at 25 ℃. The sample injection volume was 10 μL. The results indicated that Siraitic acid ⅡB had a linear relationship at the range of 0.05-0.3 mg/mL,and Siraitic acid ⅡA had a linear relationship at the range of 0.05-0.25 mg/mL. This method was accurate,stable and reproducible,while the preparation of samples was simple and detected time was short.
Keywords:Siraitic acid ⅡB  Siraitic acid ⅡA  HPLC  detemination
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