防风体细胞胚发生和发育的组织学研究

采用石蜡切片和半薄切片法研究组织培养中防风体细胞胚发生和发育的形态和结构特征,以了解体细胞再生形成胚胎结构的细胞分化和形态发生特征。结果表明:(1)胚性细胞内含有丰富的淀粉体,随细胞分裂和分化逐渐减少;(2)体细胞胚的发生发育经历了类似合子胚的各阶段;(3)多细胞原胚形成的分裂方式以平周分裂为主,并由此表现出有序生长;(4)在体细胞胚迅速发育过程中,不同阶段的体细胞胚始终与周围细胞存在明显界限;(5)胚柄发育不明显;(6)畸形胚常见,其中连体胚是胚性细胞发生密度过高造成的,而次生胚则在原来胚的基础上又产生新的胚的发生中心。

Histological study on the somatic embryogenesis and development of Saposhnikovia divaricata

Paraffin slice and semithin section are utilized to study the characteristics of morphogenesis and anatomical structure during the occurrence and development of somatic embryo in tissue culture of Saposhnikovia divaricata. The results show:(1)there are lots of starch in the embryogenetic cell,and gradually decreased accompanying the cell fission and differentiation;(2)the somatic embryogenesis and development experience all the stages of zygote embryo;(3)the multicellular embryo is developed from periclinal division primarily,and accordantly grows regularly;(4)there is distinct boundary between the different stages of somatic embryo and the cells around;(5)the suspensor isn’t obvious;(6)the abnormal joined embryo is caused by the high density of embryonic cells, and the subordinate embryo is caused by the new occurrence center produced by the original embryo.