铁皮石斛原球茎常温保存研究

以铁皮石斛原球茎为材料,通过不同培养基、蔗糖浓度、继代周期、保存时间等多种因素对原球茎在保存过程中增殖生长和分化成苗的影响,进行铁皮石斛原球茎常温保存的研究。结果表明:铁皮石斛原球茎常温((25±2)℃)保存的适宜培养基为1/2MS、蔗糖浓度为1%,继代周期可达10个月;原球茎在5年内能保持分化和增值能力,随着保存年限的增加,分化率越来越低,可通过复壮和成苗培养提高分化成苗率。

Conservation on the protocorms of Dendrobium officinale in room temperature

The effects of different basal media,sucrose concentration,subculture period and conservation time on the propagation and differentiation of protocorms of Dendrobium officinale were studied. The results showed that the suitable basal medium was 1/2 MS,the optimum sucrose concentration was 1%,and the subculture period could last for 10 months under the room temperature. The protocorms would keep differentiation and propagation abilities in five years of conservation. As the conserved time being longer,the ability of differentiation became weaker.