香蕉小茎尖培养和快速繁殖

本文报道14个香蕉品种或品系进行小茎尖离体培养繁殖无病苗。小茎尖培养在改良MS培养基中,附加BA2.0—5.0mg/l,试验结果显示,BA明显促进芽的形成和增殖,随着BA浓度的增高,形成的芽苗数也随着增多。各品种均能诱导丛生芽,但品种间的繁殖率有很大差异。低浓度的Kt或BA有利于诱导生根。培养的试管苗经检验为无病苗。

RAPID PROPAGATION AND SHOOT TIP CULTURE OF BANANAS IN VITRO

This paper reports the shoot tip culture and rapid propagation of mosaic disease-free plants in vitro of fourteen varieties of banana. The culture of their shoot tips on modified MS medium containing 2.0-5.0 mg/l BA. The results showed that BA markedly stimulated the bud formation and proliferation, the higher concentration increased the more number of bud formation. All fourteen varieties of banana induced many buds formation, but there is great difference in their porpagation coefficient. Low concentration KT or BA was beneficial to root formation. The test-tube plantlets derived from shoot tip culture were disease-free by visual inspection.