| 海南主要普通野生稻的调查及利用RAPD技术进行鉴别分析 |
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| 引用本文： | 王晓玲,郭安平,孔 华,刘恩平,贺立卡.海南主要普通野生稻的调查及利用RAPD技术进行鉴别分析[J].广西植物,2008,28(6):741-745. |
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| 作者姓名： | 王晓玲 郭安平 孔 华 刘恩平 贺立卡 |
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| 作者单位： | 1. 中国热带农业科学院,热带生物技术研究所,海口,571101;江西省农业科学院,水稻研究所,南昌,330200
2. 中国热带农业科学院,热带生物技术研究所,海口,571101 |
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| 基金项目： | 国家农业部农业结构调整重大技术研究专项，中央级公益性科研院所基本科研业务费热带区转基因植物生物安全及生物多样性研究，华南热带农业大学创新基金 |
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| 摘 要： | 通过对海南现存的主要普通野生稻资源进行考察,报道了海南主要普通野生稻的最新生存状况;并用RAPD技术对其进行分析。从供试材料中筛选出具有多态性的RAPD引物13条,共扩增出155条带,其中多态性条带120条,多态性条带比率(PPB)值为77.42%。UPGMA聚类结果表明:海南主要普通野生稻之间遗传关系较复杂、差异较明显,并将海南主要普通野生稻资源划分为5个小类群,可以很好地将海南本地野生稻与收集保存的野生稻鉴别开来,为海南普通野生稻资源的合理利用与有效保护提供了重要依据。
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| 关 键 词： | 普通野生稻 资源 RAPD标记 遗传多态性 |
Investigation, identification and analysis of main
common wild rice(Oryza rufipogon)
from Hainan Province by RAPD |
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| WANG Xiao-Ling,GUO An-Ping,KONG Hu,LIU En-Ping,HE Li-Ka.Investigation, identification and analysis of main
common wild rice(Oryza rufipogon)
from Hainan Province by RAPD[J].Guihaia,2008,28(6):741-745. |
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| Authors: | WANG Xiao-Ling GUO An-Ping KONG Hu LIU En-Ping HE Li-Ka |
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| Institution: | 1.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou
571101, China; 2.Rice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China |
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| Abstract: | Nowadays existing state of common wild rice (Oryza rufipogon) from Hainan Province was investigated in this paper. Genetic diversity of them were studied and researched by random amplified polymorphic (RAPD) primers. Total 13 RAPD primers were screened. From RAPD marker graphs,155 bands were generated,including 120 polymorphic bands,with 77.42% proportion of polymorphic bands (PPB). Clustering analysis by UPGMA showed that O.rufipogon from Hainan could be classified into 5 types. Common wild rices between local species and collected species could be identified. The model of utilizing reasonably and protecting effectly natural population of common wild rice was proposed. |
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| Keywords: | commen wild rice (Oryza rufipogon) resource RAPD Marker genetic diversity |
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