| 5℃低温对转SOD或POD基因烟草影响的研究 |
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| 引用本文： | 曾淑华,刘飞虎,.5℃低温对转SOD或POD基因烟草影响的研究[J].广西植物,2006,26(5):488-491,487. |
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| 作者姓名： | 曾淑华 刘飞虎 |
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| 作者单位： | 1. 云南农业大学烟草学院,云南,昆明,650201;云南大学生命科学学院,云南,昆明,650091
2. 云南大学生命科学学院,云南,昆明,650091 |
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| 摘 要： | 对盆栽9叶龄的3个烟草近等基因系进行5℃低温处理。测定处理2d、4d和6d以及处理6d后恢复生长2d和4d烟草叶片的生理生化指标,观察处理2d、4d和6d后恢复生长7d烟草的生长情况。结果表明随着低温处理时间的延长,细胞质膜透性、丙二醛含量、可溶性蛋白质和糖含量均升高;SOD活性则开始下降随后升高,但始终低于对照;低温造成株高、叶片数和生物量显著下降。
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| 关 键 词： | 转基因烟草 低温处理 SOD POD 生物量 |
| 文章编号： | 1000-3142（2006）05-0488-04 |
| 收稿时间： | 2004-11-01 |
| 修稿时间： | 2004-11-012005-08-07 |
A study on effects of low temperature at 5℃ on transgenic tobacco with SOD or POD over-expressed |
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| ZENG Shu-hua,LIU Fei-hu.A study on effects of low temperature at 5℃ on transgenic tobacco with SOD or POD over-expressed[J].Guihaia,2006,26(5):488-491,487. |
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| Authors: | ZENG Shu-hua LIU Fei-hu |
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| Institution: | 1. College of Tobacco Science, Yunnan Agricultural University, Kunming 650201, China; 2. College of Life Sciences, Yunnan University, Kunming 650091, China |
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| Abstract: | Three lines of near isogenic tobacco were pot-cultured and treated under low temperature(5℃)at their 9-leaf stage. Some physiological and biochemical indices were tested respectively on the 2nd,4th and 6th day of treatment and on the 2nd and 4th day of recovery growth after 6 days of treatment,and the growth of tobacco was observed on the 7th day of recovery growth after 2,4 and 6 days of treatment respectively. The results indicated that as the time of plants under low temperature elongated,cell membrane permeability,MDA content,soluble protein and sugar content increased,but the plants height,number of leaves and biomass of per plant decreased. While the SOD activity first decreased and then increased. |
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| Keywords: | transgenic tobacco low temperature SOD POD biomass |
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