广西地不容种质资源的ISSR分析

采用简单序列重复区间扩增(ISSR)分子标记技术对广西地不容3个野生居群和1个引种居群共92个个体进行了遗传多样性研究。10个引物共扩增出61条带,其中60条具多态性,多态性位点百分率为98.36%。4个居群多态性百分率在73.77%～86.89%。Nei’s基因多样性指数(H)为0.3379,Shannon信息多样性指数(Ⅰ)为0.5055。3个野生居群Nei’s遗传分化系数(Gst)表明:83.87%遗传变异分布在居群内,16.13%的遗传变异分布在居群间。引种居群与3个野生居群间的遗传一致度达0.8846。引种居群有效地保护了广西地不容的遗传多样性。

ISSR analysis on the germplasm resource of Stephania kwangsiensis

The genetic variations among the introduced population and three wild populations of Stephanis kwangsiensis were analyzed by ISSR marker.Ten primers gave rises to 61 disernibles DNA bands,of which 61(98.36%)were polymorphic,indicating a considerable genetic variation at specific level.At the population level,the percentage of polymorphic bands(PPL)was from 73.77% to 86.89%.Genetic diversity analysis showed that Nei's gene diversity(H)was 0.337% and shannon's genetic diversity index(Ⅰ)was 0.505 5.Based on Nei's Gst value of three wild populations,a large proportion of genetic variance(83.87%)was among individuals within population,while only a small proportion(16.13%)genetic variance was among populations.Genetic identity between the introduced population and three wild was 0.884 6.The introduced population effectively protected the genetic diversity of S.kwangsiensis.