荞麦属种质资源发芽种子过氧化物酶同工酶研究

用聚丙烯酰胺凝胶电泳对荞麦属9个种(含大粒组8个和小粒组1个)32个收集系栽培及野生荞麦种子的过氧化物酶同工酶进行了研究。结果表明:过氧化物酶同工酶酶带23条,不同物种的酶带数4到8条。其中,甜荞有7条带,而苦荞为4条。酶带分析及聚类分析表明:大粒组荞麦种的谱带与F.gracilipes等小粒组荞麦种间差异极大,甜荞和苦荞酶带分别与F.megaspartanium和F.pilus相似,并分别与F.megas-partanium和F.pilus聚类最近,支持F.megaspartanium和F.pilus可能分别是甜荞和苦荞祖先种的假说。

Peroxidase isozyme in sprouting seeds of genus Fagopyrum

The peroxidase isozyme of thirty-two collections of cultivated and wild buckwheat belonging to nine species of genus Fagopyrum including eight species of the big-achene group and one species of the small-achene group were studied by means of polyacrylamide gel eletrophoresis(PAGE).The results showed that there are twenty-three different bands of peroxidase isozyme,with a range from four bands to eight bands among different buckwheat species.F.esculentum and F.tataricum have seven bands and four bands among different collections,respectively.The variations of peroxidase isozyme zymograph are very great among different buckwheat species but less in the same species.The results of the zymograph analysis and the clustering showed that there are much great difference of zymographs between the big-achene group and the small-achene group and that F.megaspartanium and F.pilus are close in clustering tree to F.esculentum and F.tataricum,respectively,supporting Chen's hypothesis that F.megaspartanium and F.pilus may be the ancestors of F.esculentum and F.tataricum,respectively.