海南西海岸红树林伴生植物内生放线菌多样性及其延缓衰老活性初筛

为寻找新型抗衰老药物,该文以海南西海岸红树林伴生植物为研究对象,采用9种不同培养基从7种伴生植物21份样品中分离纯化放线菌,通过PCR扩增,16S rRNA基因序列分析已纯化放线菌的多样性,利用秀丽隐杆线虫(Caenorhabditis elegans)模型筛选菌株来进行延缓衰老活性研究。结果表明:(1)从7种伴生植物21份样品中共分离到26株海洋放线菌,隶属于9科15属,分别为拟诺卡菌属、短状杆菌属、短小杆菌属、Demequina、戈登氏菌属、类诺卡氏菌属、Lysinimicrobium、细杆菌属、假诺卡氏菌属、微球菌属、原小单孢菌属、拟无枝酸菌属、Yimella、北里孢菌属和链霉菌属,其中链霉菌属为优势菌属。(2)经秀丽隐杆线虫模型筛选,发现有2株海洋放线菌的发酵粗提物具有延缓秀丽隐杆线虫衰老的作用。综上结果说明海南西海岸红树林伴生植物中含有丰富多样的药用放线菌资源,为海洋放线菌抗衰老研究奠定了基础。

Diversity and anti-aging activity of endophytic actinobacteria from associated mangrove plants collected from west coast of Hainan

In order to search for new anti-aging drugs, actinobacteria were isolated from seven associated mangrove plants and nine different isolation media. The strain diversities were analyzed by using PCR amplification and comparison of 16S rRNA gene sequences. The anti-aging activities of the actinobacteria were analyzed by using Caenorhabditis elegans screening models. The results were as follows:(1)A total of 26 marine actinobacteria were isolated from 21 samples of seven associated mangrove plants, belonging to nine families and fifteen genera consist of Nocardiopsis, Brachybacterium, Curtobacterium, Demequina, Gordonia, Nocardioides, Lysinimicrobium, Microbacterium, Pseudonocardia, Micrococcus, Promicromonospora, Amycolatopsis, Yimella, Kitasatospora and Streptomyces, and the dominant genus was Streptomyces.(2)Two kinds of actinobacteria were found to have anti-aging effect by Caenorhabditis elegans screening models. All the above results indicate that there are abundant medical actinobacteria resources in the west coast of Hainan, and the study provides research fundamental data for anti-aging research of marine actinobacteria.