黄槐叶片培养过程中器官发生的研究

以黄槐（ＣａｓｓｉａｓｕｒａｔｔｅｎｓｉｓＢｕｒｍ．ｆ．）幼嫩叶片为材料，接种于ＭＳ＋ＮＡＡ１ｐｐｍ＋２，４－Ｄ１ｐｐｍ＋６－ＢＡ２ｐｐｍ的培养基上，诱导形成两种形态的愈伤组织，即致密愈伤组织与雪花状愈伤组织．将愈伤组织转移到ＭＳ＋ＮＡＡ：１ｐｐｍ＋６－ＢＡ２ｐｐｍ的分化培养基上．仅致密型愈伤组织经过球状体至不定芽途径形成大量再生植株。扫描电镜及组织细胞学观察表明，致密愈伤组织表层细胞排列紧密，有许多分生细胞团，而雪花状愈伤组织表层细胞薄壁化，分裂能力很低。球状体起源于致密愈伤组织表层的分生细胞团，其细胞有极强的分生能力，顶端可以分化发育成不定芽原基，最后形成不定芽并发育成小植株。球状体可以看成是具有形成不定芽能力的繁殖单位．

STUDIES ON ORGANOGENESIS OF LEAVES CULTURE OF CASSIA SURATTENSIS BURM.F.

Two kinds of calli(i.e dense callus and snowflaky callus)were produced from immature leaves of Cassfa surottensis Burm.f.on medium MS+NAA 1 ppm+2,4-D 1 ppm+6-BA 2 ppm.A latge number of plantlets were obtained from the redifferentiation of dense callus via globoid and adventitious bud when transfered on medium MS+FNAA 1 ppm+6-BA 2 ppm. With scanning electron microscope and histocytological method the observation showed that there were a lot of meristematic cell masses in the Periphery of dense callus but little in snowflaky callus.The globoid originated from meristematic cell mass on the surface of dense callus, Its top redifferentiated and formed primordium of adventitious bud.Finally,the adventitious bud developed into plantlet.Therefore,the globoid can be COnsidered as a proliferation unit producing adventitious bud.