中国石竹离体快繁与试管苗玻璃化研究

研究了培养基组成成分对中国石竹品种Diana F1 White组培程序中种子萌发、诱芽增殖、试管苗玻璃化及生根等重要环节的影响。结果表明:(1)种子适宜的萌发培养基为1/2MS,在此培养基中种子萌发率为83.33%,播种后30d时无菌苗高度达6.99cm,叶片数达26.7。(2)在芽诱导阶段玻璃化苗发生率最高可达53.85%。将光照强度提高至2000lx后,采用培养基MS+6-BA3.0mg/L+NAA0.3mg/L+琼脂8.0g/L+蔗糖8.0g/L进行诱芽培养,玻璃化苗发生率降至3.33%,外植体出芽率达到90%,单个外植体出芽数达到7.2个。(3)适宜的生根培养基为1/2MS+1.0mg/LIBA+琼脂8g/L+蔗糖40g/L,培养30d时生根率为100%,平均根条数达到24.7条,平均根长度达到4.7cm。试管苗在消毒后的腐殖土中移栽,成活率达95%。该研究结果为DianaF1试管苗的快速繁殖提供科学依据。

Study on in vitro propagation and tube plant vitrification of Dianthus chinensis

Effects of medium ingredients on seeds germination,buds inducement proliferation,tube plant vitrification and rooting were studied in order to establish efficient rapid propagation technology system of Dianthus chinensis cultivar Diana F1 White.The results showed that proper seeds germination medium was 1/2 MS,and seed germination ratio was 83.33%.Height of aseptic tube plant reached to 6.99 cm,and leaf number per plant was 26.7 on 30th day after sowing in medium.Max.vitrification plant ratio was 83.33% during bud inducement period for tube plant of Diana F1 White.The optimal medium for buds inducement was MS+6-BA 3.0 mg/L+NAA 0.3 mg/L+agar 8.0 g/L+sugar 8.0 g/L after light intensity was promoted to 2 000 lx.Bud inducement ratio and buds number per explant were 90% and 7.2 respectively,and the ratio of vitrification tube plants decreased to 3.33% after 30 days’culture.The optimal rooting medium was 1/2 MS+1.0 mg/L IBA+agar 8 g/L+sugar 40 g/L,and rooting percent,roots number and average root length was 100%,24.7 and 4.7 cm respectively after 30 days’ culture.Survival rate of tube plant was more than 95% after they were transplanted in disinfected humus.The above results would provide scientific base for micro propagation of D.chinensis cultivar Diana F1 White.