| 黄花蒿中青蒿素含量的RP-HPLC法测定 |
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| 引用本文： | 刘金磊,李典鹏,韦,霄,黄永林,卢凤来,蒋运生.黄花蒿中青蒿素含量的RP-HPLC法测定[J].广西植物,2007,27(5):808-810. |
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| 作者姓名： | 刘金磊 李典鹏 韦 霄 黄永林 卢凤来 蒋运生 |
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| 作者单位： | 广西壮族自治区,中国科学院,广西植物研究所,广西,桂林,541006 |
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| 基金项目： | 国家自然科学基金(30660222)，广西科技攻关项目(0663003)，中国科学院农办项目~~ |
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| 摘 要： | 建立黄花蒿中青蒿素的高效液相色谱测定方法,采用柱前衍生RP-HPLC法测定黄花蒿中青蒿素的含量,采用ZORBAXXDB-C18(4.6mm×150mm,5μm)色谱柱,甲醇-0.01mol/L醋酸钠-醋酸缓冲液(pH5.8,体积比62∶38)为流动相;检测波长:260nm;流速:0.8mL/min;柱温:30℃。对广西、沈阳、北京、郑州、苏州和杭州等不同产地的野生黄花蒿样品、以及同一产地不同采集时间黄花蒿样品进行检测,结果表明不同地区青蒿素含量差异很大,同一地区不同采集时间黄花蒿样品的青蒿素含量也有差异。该法准确可靠、重现性好,能准确地反映青蒿素含量的检测。
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| 关 键 词： | RP-HPLC 黄花蒿 青蒿素 含量测定 |
| 文章编号： | I000-3142（2007）05-0808-03 |
| 修稿时间： | 2007-02-092007-06-16 |
Determination of artemisimin in Artemisia
annua with RP-HPLC |
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| LIU Jin-Lei,LI Dian-Peng,WEI Xiao,HUANG Yong-Lin,LU Feng-Lai,JIANG Yun-Sheng.Determination of artemisimin in Artemisia
annua with RP-HPLC[J].Guihaia,2007,27(5):808-810. |
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| Authors: | LIU Jin-Lei LI Dian-Peng WEI Xiao HUANG Yong-Lin LU Feng-Lai JIANG Yun-Sheng |
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| Institution: | Guangxi Institute of Botany, Guangxi Zhuangzu Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China |
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| Abstract: | The content of artemisimin in Artemidia annua was determined with the method of pre-column reaction to RP-HLPC. The column was ZORBAXXDB-C18(4.6 mm×150 mm,5 μm),with mobile phase CH3OH-0.01 mol/L NaAC-HAC buffer(pH=5.8,62∶38),flow rate 0.8 mL/min,column temperature at 30 ℃. The results showed that there are great diversity in the content of artemisimin in Artemidiae annua from different places and different harvest time. The method is accurate,sensible and repeatable,and can be used for the quality control of this herbal medicine. |
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| Keywords: | RP- HPLC Artemisia annua L artemisimin determination |
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