蛇苔过氧化物酶基因的序列分析与功能预测

过氧化物酶在生物界分布极广,在细胞代谢的氧化还原过程中起重要的作用.为深入研究蛇苔过氧化物酶的理化性质及功能,对其同源性,亚细胞定位,结构和功能等进行了生物信息学预测和分析.结果表明；该序列有1 050 bp长的开放阅读框,编码349个氨基酸,与拟南芥等的POD相似性较高(相似性为71％).预测R68、F71、H72、...

Sequence analysis and function predication of poroxidase from Conocephalum conicum

Peroxidases is widespread in biological world.It plays an important role in the cell redox metabolism.In order to furher study the characteristics and functions of Conocephalum conicum poroxidase,we performed bioinformatics analysis of its homology,subellular location,tertiary structure,function and so on.The results showed that the sequence had a complete open read frame of about 1 050 bp and encodes 349 amino acids,and the sequence had similar POD with that of Arabidopsis thaliana(the similarity beween them is 71%).This sequence was predicated to be C.conicum poroxidase which belonged to the poroxidase super family,and R68、F71、H72、P170、H200 and D276 to be the C.conicum POD's active sites in which the four groups of disulfide bonds:C4 and C122、C74 and C79、C128 and C328、C207 and C239 played a very important role in maintaining the protein's function,natural conformation and stability.This study would set an important foundation for the study of the interaction mechanisms and functions of the C.conicum poroxidase.