基于LC-MS代谢组学技术的丽豆-大豆差异代谢物比较研究

丽豆(Calophaca sinica)是我国华北地区特有的一种珍稀野生植物。为探明丽豆的营养价值，该文以大豆(Glycine max)为参照组，利用液相色谱-质谱联用(LC-MS)技术对其种子进行了比较代谢组学研究。结果表明：(1)丽豆和大豆中共检测到1 857种代谢产物，二者成分相同且含量相似的代谢物有1 698种(>90%),差异代谢物有159种(<10%)。(2)在差异代谢物中，成分差异的有9种，其中有5种为丽豆特有，剩余150种均为含量差异，其中48种(约30%)在丽豆中的含量高于大豆。(3) KEGG注释到8条差异代谢物显著富集(P<0.1)的通路，主要包括初生代谢物的各类氨基酸生物合成途径和次生代谢物的罗汉松脂素、花生四烯酸以及二萜类等生物合成途径。(4)丽豆比大豆含量低的化学组分主要是初生代谢产物，比大豆含量高的化学组分主要是次生代谢物，而这些次生代谢物在调节血糖、骨坏损修复、增强免疫以及消炎抗癌等生理过程中有着积极的作用。综上所述，该研究认为丽豆与大豆具有相近的营养价值，并且对改善人类亚健康状况有积极的影响；此外，该文使我们对丽豆的营养价值和代谢组成...

Comparative analysis of differential metabolites between Calophaca sinica and Glycine max based on LC-MS metabolomics technology

Calophaca sinica is a rare wild plant endemic to North China. To explore the nutritive value of C. sinica, the comparative metabolomics of C. sinica and Glycine max(as control group)seeds were studied by liquid chromatography-mass spectrometry(LC-MS). The results were as follows:(1)A total of 1 857 metabolites were detected in these two species, and 1 698 metabolites were similar in composition and content(>90%), and only 159 differential metabolites(<10%).(2)Among the metabolites, nine were different in components, of which five were unique to C. sinica, and the others 150 were different in content, of which 48 chemical components(about 30%)were higher in C. sinica than in Glycine max.(3)Eight pathways were significantly enriched with differential metabolites(P<0.1)by KEGG annotation, and it mainly including the biosynthesis pathways of primary metabolite(various amino acid)and secondary metabolites(such as matairesinol, arachidonic acid and diterpenoids).(4)Among these statistically significant differential chemical components, of which lower content in Calophoca sinica are mainly primary metabolites, and higher content are mainly secondary metabolites. These secondary metabolites play an active role in regulating blood glucose, repairing bone damage, enhancing immunity, anti-inflammatory and anti-cancer. In summary, the result supports that the nutritional value of C. sinica is similar to that of Glycine max, and it also has a positive effect on improving human sub-health status. Meanwhile, this study provides us a relatively comprehensive understanding of the nutritive value and metabolic components of Calophaca sinica and necessary data for the deep development and efficient utilization of C. sinica resources in future.