樟叶越桔组培苗生根和移栽技术研究

为解决樟叶越桔(Vaccinium dunalianum)组培苗生根质量不佳、移栽成活率低的问题,该研究以樟叶越桔继代苗为材料,采用单因子试验从激素类型及浓度、培养基类型和蔗糖质量浓度对其生根的适宜条件进行筛选,进一步研究了不同基质配比对樟叶越桔移栽苗存活率的影响。结果表明:激素类型和浓度、培养基类型对樟叶越桔生根率的影响最大,其次为蔗糖质量浓度;最适合樟叶越桔生根的激素及浓度为IBA2.0 mg·L~(-1)、基本培养基类型为1/4MS、蔗糖质量浓度为15 g·L~(-1),樟叶越桔组培苗最佳生根培养基为1/4MS+IBA 2.0 mg·L~(-1)+活性炭0.1 g·L~(-1)+蔗糖15 g·L~(-1),生根率达100%,平均生根数为每株7.67条;根系呈辐射状、基部无愈伤组织,组培苗生长健壮、叶色浓绿;樟叶越桔组培苗移栽时以全腐殖土基质为佳,成活率为83.7%,植株叶片舒展,生长状况良好。该研究建立的优化体系有效地提高了樟叶越桔组培生根苗的生根率和生根质量,解决了后期移栽成活困难的问题,为优良的樟叶越桔植株规模化生产提供了科学依据和技术支持。

Rooting and transplanting techniques of tissue culture plantlets of Vaccinium dunalianum

Vaccinium dunalianum is one of the main plant sources for arbutin which is widely used in cosmetic formulations as the natural active agents to whiten the skin. During the tissue culture process of V. dunalianum, the base of the rooting plantlets obtained by the current culture medium often formed the callus, which significantly affected the survival rate after transplantation. In order to improve the rooting quality of the proliferation plantlets and survival rate of the transplanted plantlets of V. dunalianum. The optimum medium components for the root induction of the proliferation plantlets of V. dunalianum, including hormone types and concentrations, medium types and sucrose concentrations, were analyzed by the single factor experiment, and further probed the effects of different substrate ratios on the survival rate of the transplanted V. dunalianum plantlets in this study. The results showed that the medium types and the hormone types and their concentrations had more significant effects on the rooting rate than sucrose concentration. For the root induction, the optimal hormone and its concentration were IBA 2.0 mg·L^-1, the optimal media was 1/4MS and the optimal sucrose concentration was 15 g·L^-1. The most suitable medium for the root induction of the proliferation plantlets of V. dunalianum was 1/4MS+ IBA 2.0 mg·L^-1+ activated carbon 0.1 mg·L^-1+ sucrose 15 g·L^-1, by which the rooting rate could reach 100%, and the average number of roots was 7.67 per plant. The root system of proliferation plantlets of V. dunalianum was radially extended and none callus was formed at the base of the rooting plantlets. In addition, the tissue culture plantlets of V. dunalianum grew strong and had dark-green leaves. For the transplantation of V. dunalianum plantlets, the optimal media for transplantation was the 100% humus, in which the survival rate reached 83.7%, and the tissue culture plantlets obtained by the experiments grew well and had healthy leaves. The optimization system established by this study, which had effectively improved the roots quality and rooting rate of the proliferation plantlets and the survival rate of the transplanted plantlets of V. dunalianum, and provided scientific and technological support for the large-scale production of high quality V. dunalianum plants.