两面针基原植物考证及其活性成分含量分析

该研究选取广西不同类型的两面针为材料,通过查阅文献对两面针基原植物进行考证,用反相高效液相色谱法(RP-HPLC)对两面针原变种及变种中的5种活性成分进行定量分析。结果表明:(1)毛两面针Zanthoxylum nitidum (Roxb) DC. f. fastuosum How ex Huang]早已并入两面针原变种Z. nitidum (Roxb.)DC. var. nitidum];虽然毛两面针与毛叶两面针中文名称相似,但不可认为是同一个变种。(2) RP-HPLC结果显示两面针原变种及变种均不含毛两面针素。(3) 5种活性成分在不同类型中存在显著性差异。(4)根据5种活性成分含量数据,对9份两面针样品进行聚类分析,结果划分为4组,与两面针形态划分一致。这不仅理清了"毛叶两面针"与"毛两面针"存在的混淆问题,而且还比较了不同形态类型间的活性成分含量,为两面针质量控制和资源的合理应用开发提供了参考。

Botanical origin research and simultaneous determination of five effective components in Zanthoxylum nitidum

In this study, different phenotypic types of Zanthoxylum nitidum from Guangxi were selected to illustrate the original scientific name of Zanthoxyli Radix through literature review and conduct the quantitative analyses of five effective components based on RP-HPLC. The results were as follows:(1)Zanthoxylum nitidum f. fastuosum that shared the similar Chinese name with Z. nitidum var. tomentosum but was distinctly different between each other had already been incorporated into Z. nitidum var. nitidum;(2)Toddaloactone was not included in both Z. nitidum var. nitidum and Z. nitidum var. tomentosum;(3)The content of effective components in different types was significantly distinct;(4)Clustering analysis based on the content of five effective components separated all populations into four groups, corresponding to phenotypic delimitation. Totally, this study not only clarified the confusion between the Z. nitidum var. tomentosum and Z. nitidum var. nitidum Type 3, but also illustrated the significant difference of effective components in four phenotypes. All results provide guidance for quality control and the rational application and development of resources in Z. nitidum.