柬埔寨柯拉斯那沉香的化学成分研究

该文采用ODS、硅胶、Sephadex LH-20等柱色谱技术,对柬埔寨野生柯拉斯那沉香(Aquilaria crassna)进行了研究。结果表明:从柬埔寨柯拉斯那所产沉香的乙醇提取物中进行分离共得到了10个化合物,包括一对对映异构体(9a/9b)。经波谱解析分别鉴定为6-甲氧基-2-2-(3-羟基-4-甲氧基苯)乙基]色酮(1)、6-甲氧基-2-2-(3-甲氧基-4-羟基苯)乙基]色酮(2)、6,7-二甲氧基-2-(2-苯乙基)色酮(3)、6-羟基-2-(2-苯乙基)色酮(4)、6-羟基-2-2-(4-甲氧基苯)乙基]色酮(5)、8-氯-6-羟基-2-2-(3-羟基-4-甲氧基苯)乙基]色酮(6)、8-氯-6-羟基-2-2-(4-甲氧基苯)乙基]色酮(7)、oxidoagarochromone B(8)、4'-demethoxyaqusisnenone D(9)。其中,化合物6、7和9均为首次从柯拉斯那沉香中分离得到。活性测试结果显示,化合物1和2对乙酰胆碱脂酶具有一定的抑制活性,化合物2对人慢性髓原白血病细胞K562具有较弱的抑制作用。

Chemical constituents of agarwood originating from Aquilaria crassna in Cambodia

Ten compounds, including one pair of enantiomers(9a/9b), were isolated from the ethanol extract of agarwood originating from Aquilaria crassna in Cambodia by comprehensive chromatographic techniques including ODS, silica gel and Sephadex LH-20. On the basis of spectroscopic data, they were identified as 6-methoxy-2- 2-(3-hydroxy-4-methoxyphenyl)ethyl] chromone(1), 6-methoxy-2- 2-(3-methoxy-4-hydroxyphenyl)ethyl] chromone(2), 6,7-dimethoxy-2-(2-phenylethyl)chromone(3), 6-hydroxy-2-(2-phenylethyl)chromone(4), 6-hydroxy-2- 2-(4-methoxyphenyl)ethyl ] chromone(5), 8-chloro-6-hydroxy-2- 2-(3-hydroxy-4-methoxyphenyl)ethyl]chromone(6), 8-chloro-6-hydroxy-2- 2-(4-methoxyphenyl)ethyl] chromone(7), oxidoagarochromone B(8), 4''-demethoxyaqusisnenone D(9). Compounds 6, 7 and 9 were isolated from agarwood of A. crassna for the first time. Among those, compounds 1 and 2 showed inhibitory activities against acetylcholinesterase. Compound 2 had weak inhibitory effect on K562 cell lines.