6-BA、IBA、2;4-D对杖藤组培增殖的影响

研究了6 BA、IBA和2,4 D对杖藤组培物增殖的影响。结果表明,6 BA对增殖芽数、丛芽率和母芽高均有显著的作用,IBA和2,4 D仅对增殖芽数有显著影响。适宜于增殖培养的激素浓度分别为6 BA4.0mg/L,IBA0.25mg/L,2,4 D0.25mg/L。以此激素浓度组合,连续增殖培养6～7代后,平均的增殖芽数可稳定在10个左右。

Influence of 6-BA,IBA and 2,4-D on proliferation of Calamus rhabdocladus

This paper deals with the influence of 6 BA(6 benzylaminopurine),IBA(indolebutyric acid)and 2,4 D(2,4 dichlorophenoxyacetic acid) on in vitro proliferation of Calamus rhabdocladus Burret. The experimental results show that 6 BA has eminent influence on proliferation rate of explants,shoot amount proliferated per explant and shoot height. IBA and 2,4 D only have eminent influence on shoot amount proliferated per explant. The appropriate concentrations of 6 BA,IBA and 2,4 D in proliferation medium of C. rhabdocladus are 4.0 mg/L,0.25 mg/L and 0.25 mg/L respectively. After seven subcultures on medium supplemented with appropriate concentration of plant growth regulators,shoot amount proliferated per propagule can reach as high as 11.33.