罗汉果组培繁殖的技术要点

报道罗汉果组培繁殖的各项主要技术要点,包括组培条件、培养基的配制、外植体的选取与消毒、接种与培养、种源保存、炼苗与移栽、苗木包装与运输等。提出了5种培养基参考配方,即茎段诱导培养:MS+BA0.5～1.0mg/L+IAA(NAA)0.05～0.1mg/L+白糖3%+琼脂4.5g/L,pH5.8;茎尖诱导培养:MS+BA0.5～1.0mg/L+NAA0.05～0.1mg/L+椰子水100mL+白糖3%+琼脂4.5mg/L,pH5.8;继代培养(丛生芽方式):MS+BA0.3～0.7mg/L+NAA0.05/IAA0.1mg/L+白糖3%+琼脂4.5mg/L,pH5.8;继代培养(微型扦插方式):MS+BA0.1mg/L+IAA0.3mg/L+活性炭0.07g/L+白糖3%+琼脂4.5mg/L,pH5.8;生根培养:MS+BA0.07mg/L+IBA0.15mg/L+IAA0.1mg/L+活性炭0.1g/L+白糖3%+琼脂4.5mg/L,pH5.8。分析了外植体培养过程中可能出现的不良状况的原因并提出预防措施,明确了炼苗移栽的适宜条件并制定出相应的管理方法。形成了一套较为完整的罗汉果组培苗繁殖生产技术规程。

Technology procedure of Tissue culture and Propagation of Siraitia grosvenorii

The primarily technology of tissue culture and propagation of Siraitia grosvenorii was reported,contains the condition of tissue culture,confection of medium,selection and sterilization of explant,inoculation and culture,preservation of superior provenance,hardening-seedling and transplanting,plant pack and transportation. And 5 kinds of referenced medium were reported too. The medium of induced stem was MS+BA0.5～1.0 mg/L+IAA(NAA)0.05～0.1 mg/L+sugar 3%+agar 4.5 g/L,pH5.8. The medium of induced stem apex was MS+BA0.5～1.0 mg/L+NAA0.05～0.1 mg/L+coconut milk 100 mL+sugar 3%+agar 4.5 mg/L,pH5.8. The medium of subculture of fasciculate bud was MS+BA0.3～0.7 mg/L+NAA0.05/IAA0.1 mg/L+sugar 3%+agar 4.5 mg/L,pH5.8. The medium of subculture of cutting was MS+BA0.1 mg/L+IAA0.3 mg/L+active carbon 0.07 g/L+sugar 3%+agar 4.5 mg/L,pH5.8. The rooting medium was MS+BA0.07 mg/L+IBA0.15 mg/L+IAA0.1 mg/L active carbon 0.1 g/L+sugar 3%+agar 4.5 mg/L,pH5.8. On the other hand,the cause of ill phenomenon in the process of tissue culture was analyzed,preventive measure was put forward. And feasible condition of hardening-seedling and transplanting was clear,the relevant manage method was formed. The integrated technology procedure of tissue cultured plantlets of S.grosvenorii was established.