几种生理因子对印楝细胞悬浮培养生长的影响

通过研究不同生理因子对印楝悬浮细胞生长的影响 ,建立了一种快速生长的印楝细胞悬浮培养体系。结果表明 ,在添加了 6 BA 0 .8mg/L、NAA 0 .8mg/L、蔗糖 3 0 g/L且初始 pH值为 5 .8的MS培养液中 ,以 60g/L的接种量进行印楝细胞的悬浮培养 ,细胞生长速率可达 4.49g/L·d。

Effects of several physiological factors on the growth of cell suspension culture of Azadirachta indica

Effects of several physiological factors on the growth of cell suspension culture of Azadirachta indica was studied and a fast growing cell suspension culture system of Azadirachta indica was established. The result showed that the cell growth rate of Azadirachta indica could reach 4.49 g/L·d under the following physiological conditions: the cell cultured on a Murashige and Skoog (MS) medium supplemented with 6-BA 0.8 mg/L,NAA 0.8 mg/L and sucrose 30 g/L,with the initial pH 5.8 in the medium and the cell amount of inoculation 60 g/L of fresh cells.