| 桑树肌动蛋白actin基因全长序列的克隆与分析 |
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| 引用本文： | 孔卫青,杨金宏.桑树肌动蛋白actin基因全长序列的克隆与分析[J].广西植物,2012,32(3):362-366. |
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| 作者姓名： | 孔卫青 杨金宏 |
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| 作者单位： | 孔卫青 (安康学院陕西省蚕桑重点实验室,陕西安康,725000) ； 杨金宏 (安康学院陕西省蚕桑重点实验室,陕西安康,725000) ； |
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| 基金项目： | 陕西省教育厅科技计划项目 |
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| 摘 要： | 肌动蛋白在植物的各种生理活动中起着重要作用,是研究基因表达与调控模式的内标参考。通过染色体步移方法获得了桑树肌动蛋白actin基因1612bp的序列,该基因CDS长1312bp(GenBank登录号:HM623866),编码377个氨基酸残基,与水稻、葡萄等的同源基因的序列一致性在90%以上。基因内含子的数目及其在基因组上的位置也与水稻、葡萄等的相似。对来自不同物种的24个肌动蛋白基因进行聚类分析的结果显示,基因被分为ClassⅠ和ClassⅡ两个明显的亚群。
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| 关 键 词： | 桑树 肌动蛋白 染色体步移 克隆 序列分析 |
Cloningand sequence analysis of full length of mulberry(Morus alba) actin gene |
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| KONG Wei Qing,YANG Jin Hong.Cloningand sequence analysis of full length of mulberry(Morus alba) actin gene[J].Guihaia,2012,32(3):362-366. |
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| Authors: | KONG Wei Qing YANG Jin Hong |
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| Institution: | (Key Sericultural Laboratory of Shaanxi,Ankang University,Ankang 725000,China) |
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| Abstract: | As a standard reference model of gene expression and regulation,actins play important roles in plants.In this study,the full length 1 612bp of actingene from mulberry was cloned by means of chromosome walking.Its CDS was 1 312bp in length,encoding 377amino acid residues.Homology analysis of the deduced amino acids showed above 90%identity with the actins from rice and grape,et al.The number and site of introns was conservative with rice and grape,too.Cluster analysis of actins from 24species indicated that these genes were divided into two subgroups of Class I and ClassⅡ. |
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| Keywords: | Morus alba actin chromosome walking cloning sequence analysis |
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