| 长柄双花木愈伤组织诱导及植株再生 |
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| 引用本文： | 曾建军,肖宜安,孙敏,.长柄双花木愈伤组织诱导及植株再生[J].广西植物,2006,26(6):628-630,601. |
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| 作者姓名： | 曾建军 肖宜安 孙敏 |
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| 作者单位： | 1. 井冈山学院,生命科学学院,江西,吉安,343009
2. 西南师范大学,生命科学学院,重庆,400715 |
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| 基金项目： | 江西省教育厅科研基金
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国家自然科学基金 |
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| 摘 要： | 以长柄双花木当年生嫩梢上的叶柄、嫩茎、嫩叶为外植体,对影响长柄双花木愈伤组织诱导和继代、分化主要因素进行研究。结果表明:在培养基MS+NAA0.5mg/L+2,4-D2.0mg/L上,三种外植体均可诱导出愈伤组织,其中叶片愈伤组织诱导率最高。该培养基还可作为愈伤组织继代培养基,但继代培养周期不超过2周。愈伤组织接种在MS+BA2mg/L上分化不定芽,根的诱导在1/2MS+IBA0.5mg/L培养基上进行。
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| 关 键 词： | 长柄双花木 愈伤组织 诱导 继代培养 |
| 文章编号： | 1000-3142（2006）06-0628-03 |
| 收稿时间： | 2005-03-14 |
| 修稿时间： | 2005-03-142005-10-20 |
Callus induction and plant regeneration of Disanthus cercidifolius var.longipes |
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| ZENG Jian-jun,XIAO Yian,SUN Min.Callus induction and plant regeneration of Disanthus cercidifolius var.longipes[J].Guihaia,2006,26(6):628-630,601. |
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| Authors: | ZENG Jian-jun XIAO Yian SUN Min |
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| Abstract: | The stems,leaves and petioles explants from Disanthus cercidifolius Maxim.var.longipes H.T.Chang were cultured on MS medium with BA,NAA,2,4-D,IBA of different concentration to induce regeneration plant. The results indicated that leaf was the best explant for callus induction,and the best suitable medium was MS NAA 0.5 mg/L 2,4-D 2.0 mg/L,it also could be used as the medium for callus subculture. MS medium only supplemented with BA mg/L could obtain higher frequency of differentiation. When studying root regeneration culture,1/2MS medium supplemented with IBA 0.5 mg/L had better root regeneration. |
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| Keywords: | Disanthus cercidifolius var longipes callus induction subculture |
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