影响魔芋愈伤组织形成的几个因素

研究了魔芋不同外植体类型、激素种类和激素组合、光暗培养条件、不同生理时期取材等因素对愈伤组织诱导能力的影响以及不同处理所得愈伤组织的分化能力。结果表明 ,不同外植体类型诱导愈伤组织能力顺序为 :顶花芽 >幼嫩芽鞘 >子芋 >根状芋 ;经过 4℃低温预处理的子芋和根状芋比未经预处理的外植体更易诱导出愈伤组织 ;MS +BA1 .0～ 2 .0 +NAA1 .0～ 2 .0培养基均能诱导外植体产生愈伤组织 ,愈伤组织诱导率大多在 5 0 %以上 ,最高达 87.5 % ;在魔芋生长期取材比在休眠期取材更易诱导出愈伤组织 ;不同处理所得愈伤组织在分化能力上具有较明显的差异。

The factors influencing on the induction of callus in Amor phophallus

Effects of several factors such as different explants,hormones,mediums and physiological periods of explants on the induction of callus in Amor phophallus and differentiation ability of callus from different treatments were studied. Results showed that the apical blossom bud was the best explants for inducing to form callus,next was the infancy bract,the daughter corms and root corms could also be induced but they had low induced rate. The daughter corms and the root corms treated by low temperature at 4 ℃ had better induced effect than the fresh explants. Four explants were all inducted to form callus in media such as MS+NAA1.0～2.0 mg/L+BA1.0～2.0 mg/L,the induced rate was mostly above 50% and the highest ratio was 87.5%. Results also showed that the explants gathering at growth period were more easy to be induced to form callus than that of gathering at dormancy period. There was distinct difference between callus induced from different explants and induced medium.