沙棘果油对过氧化氢诱导氧化损伤的保护作用

沙棘(Hippophae rhamnoides)是一种具有药用价值的植物,沙棘果油具有抗氧化、抗炎症及抗肿瘤等多种药理作用。为了探讨沙棘果油对H₂O₂造成氧化性损伤的细胞生长的影响及其抗氧化性,该研究选择H₂O₂对RAW264.7细胞氧化损伤模型,通过DPPH(1,1-二苯基-2-三硝基苯肼)自由基清除实验检测沙棘果油体外抗氧化能力,用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐]MTT法和流式细胞仪检测超氧化物阴离子荧光探针(DHE)信号,分别检测不同浓度沙棘果油对H₂O₂损伤细胞的存活率和超氧化物阴离子水平。结果表明:(1)在DPPH自由基清除实验中,当沙棘果油浓度小于4.9%时,沙棘果油的抗氧化能力大于维生素C。(2)通过MTT法发现,浓度为3.125%的沙棘果油对H₂O₂损伤细胞的存活率显著升高(P<0.01)。(3)从DHE检测发现,在同一检测时间点,随着沙棘果油浓度增加,DHE阳性细胞比例显著下降(P<0.01); 在不同检测时间点,随着沙棘果油浓度增加,DHE阳性细胞比例显著升高(P<0.01)。沙棘果油对过氧化氢诱导的RAW264.7细胞氧化损伤模型有一定修复作用,可能与细胞内超氧化物阴离子水平受到抑制有关,它具有抗氧化性损伤的潜能。

Protective effects of sea buckthorn fruit oil on oxidative damage induced by hydrogen peroxide

The sea buckthorn fruit(Hippophae rhamnoides)oil is medically claimed as having phytoantioxidant, antiinflammatory, and anticancerous properties in humans. To explore the hydrogen peroxide of the sea buckthorn fruit oil on mouse monocyte macrophages(RAW264.7 cells)oxidative damage model. By spectrophotometry, scavenging effects of sea buckthorn fruit oil on DPPH free radicals; In vitro antioxidant activity was assessed on RAW264.7 cells against hydrogen peroxide(H₂O₂)induced cytotoxicity. The antioxidant effect was determined by measuring the cell dye MTT3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] reducing assay. Then the survival rate of RAW264.7 cells was determined by MTT assay, and the expression of superoxide anion was determined by flow cytometry labeling with dihydroethidium(DHE). The results were as follows:(1)Compared with the antioxidant capacity of vitamin C, when the concentration of sea buckthorn fruit oil was less than 4.9%, the antioxidant capacity of sea buckthorn fruit oil was higher than vitamin C.(2)Comparing to the H₂O₂ group, 3.125% sea buckthorn fruit oil group had higher survival rate(P<0.01).(3)Comparing to the H₂O₂ group, the proportion of DHE-positive cell was significantly decreased in the same time(P<0.01)and it was negative effect with concentration of sea buckthorn fruit oil, the proportion of DHE-positive cell was significantly increased at the same concentration(P<0.01), and which was related positive effect with concentration of sea buckthorn fruit oil(P<0.01). These results support the protective effects of sea buckthorn fruit oil against hydrogen peroxide-induced toxicity in vitro with the underlying mechanisms of inhibiting oxidative stress. Taken together, these results clearly indicate that sea buckthorn fruit oil has significant potential as a natural antioxidant agent, which could be a potential source for the discovery of natrual antioxidant health product.