沙田柚花柱蛋白对花粉管生长的影响

本文以沙田柚及其授粉树种酸柚为材料，对其花粉在不同培养介质中的萌发率进行测定，筛选了０２～０４ｍｏｌ／Ｌ聚乙二醇－４００（ＰＥＧ－４００）＋００１％硼酸作为花粉萌发介质。用授粉后３ｄ的花柱提取液进行沙田柚、酸柚花粉萌发抑制实验，花柱提取液对自交花粉萌发无抑制作用，明显地抑制花粉管的生长。其中以花柱提取液的３５％饱和度硫酸铵级分对花粉管伸长的抑制最明显。模拟了花粉在雌蕊中的生长方式，进行花粉萌发生长，能收集到较多的花粉管，为研究花粉管的Ｓ－糖蛋白提供了材料。

Effects of the stylar proteins on the pollentube growth of shatinyu

The pollen germination rates of shatinyu (Citrus grandis varshatinyu Hort.) and its pollinating plant common pomelo, in the various culture medium were measured0.2～0.4 mol/L PEG-400 and 0.01% H3BO3 was the culture medium for the pollen germinationThe inhibitive experiments of the pollen germination of the two materials were carried on within the stylar extracting solutions in the third day after pollinationThe solution didn′t inhibite the germination of the self-pollinated pollen, but it obviously inhibited the growth of the pollen tubeThe solution (at 35% ammonium bisulphate staging) very obviously inhibited the extension of the pollen tubeThe growth ways of the pollen in the pistil were initated, and the pollen germinated and grewThe pollen tubes were collected for the researches about S-glycoproteins in there.