主要营养成分对悬浮培养玫瑰茄细胞生长和花青素合成的影响

本文研究了培养基中碳源和氮源变化对悬浮培养玫瑰茄细胞生长和花青素合成的影响。在８种不同的碳源中，麦芽糖有利于花青素的积累，而蔗糖和葡萄糖适合细胞生长，并有较高的花青素产率。在１％～１０％蔗糖浓度范围内，４％浓度下细胞生长和花青素产率最高，而６％浓度下细胞花青素含量最高，高渗环境较有利于细胞花青素的积累。１３５ｍＭ的氮源总量已足够维持玫瑰茄细胞生长和花青素合成，氮源总量增加对细胞代谢有抑制作用。ＮＨ＋４对细胞有显著抑制作用。总量１３５ｍＭ，ＮＯ－３与ＮＨ＋４比例２５∶２和２３∶４时细胞生长和花青素合成最佳。

Effects of staple nutrient on cell growth and anthocyanin production in suspension culture of Hibiscus sabdariffa L

Effects of different carbon and nitrogen source on cell growth and anthocyanin production in suspension culture of Hibiscus sabdariffa L were studied In eight kinds of carbon source, maltose was more effective toward formalasing anthocyanin content, but cell biomass and total anthocyanin production yield were highest in the medium with sucrose and glucose In the range of 1%～10% sucrose concentration, highest biomass and total anthocyanin yield were obtained in medium with 4% sucrose concentration, while highest anthocyanin content was achieved in the medium with 6% sucrose concentration High osmotic potential was effective for accumulation of anthocyanin Different total nitrogen concentration and NO - 3 to NH + 4 ratio showed strong influence on cell growth and anthocyanin production Total nitrogen concentration of 13,5 mM was enough for cell growth and anthocyanin production, while increase of the total nitrogen concentration inhibited the cell metabolism NH + 4 was a strong inhibitor Under the following condition of the ratio of NO - 3 to NH + 4 (25∶2, 23∶4) and total nitrogen concentration 13 5 mM, resulted good cell growth and anthocyanin production