HPLC法测定藜豆中左旋多巴的含量

采用HPLC法建立测定藜豆中左旋多巴含量的方法。色谱柱为岛津C-18甲基硅烷柱(250 min×4.6mm,5μm),流动相为甲醇:0.1 mol／L醋酸溶液(25:75,v／v),以硫唑嘌呤为内标物,检测波长280 nm,流速1.0 mL／min,柱温为室温,供试品的前处理方法为微波辅助提取法。结果表明,该方法准确度高,线性关系好,重现性好,平均加样回收率为97.34％,RSD 1.01％(n=5),结果满意。

Determination of Levodopa in Mucuna macrocarpa Wall by HPLC

To establish a method for determination of Levodopa in Mucuna macrocarpa Wall by HPLC. Using HPLC with Shimadzn C-18 column(250 mm×4. 6 mm,5μm),the mixture of methanol and 0. 1 mol/L acetic acid(25 : 75,v/v)as mobile phase,detection wavelength is 280nm;Azathioprine was used as internal standard substance,flow rate at 1. 0 mL/min,column temperature at r. m. The method has a good linear dependence. The average recovery of loading sample was 97. 34% with RSD 1. 01% (n = 5). The method is fast,simple and stable. It has been proved to be a good practice for determination of Levodopa in M. macrocarpa Wall.