| L-亮氨酸发酵培养基优化试验 |
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| 引用本文： | 伍时华,徐雅飞,张健,廖兰,黄翠姬,.L-亮氨酸发酵培养基优化试验[J].广西植物,2006,26(6):692-696,701. |
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| 作者姓名： | 伍时华 徐雅飞 张健 廖兰 黄翠姬 |
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| 作者单位： | 广西工学院,生物与化学工程系,广西,柳州,545006 |
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| 基金项目： | 广西科学基金项目(GKQ0229012)资助~~ |
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| 摘 要： | 应用Plackett-Burman设计试验从L-亮氨酸基础发酵培养基的10种成分中筛选出5种重要成分,然应用响应面分析试验确定5种重要成分的最适用量。培养基优化后摇瓶分批发酵72h产L-亮氨酸18.05g/L
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| 关 键 词： | L-亮氨酸 发酵培养基 优化 响应面分析 |
| 文章编号： | 1000-3142（2006）06-0692-05 |
| 收稿时间： | 2006-06-21 |
| 修稿时间： | 2006-06-212006-10-09 |
Study on medium optimization of L-leucine fermentation |
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| WU Shi-hua,XU Ya-fei,ZHANG Jian,LIAO Lan,HUANG Cui-ji.Study on medium optimization of L-leucine fermentation[J].Guihaia,2006,26(6):692-696,701. |
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| Authors: | WU Shi-hua XU Ya-fei ZHANG Jian LIAO Lan HUANG Cui-ji |
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| Institution: | Department of Biological and Chemical Engineering, Guangxi University of Technology, Liuzhou 545006, China |
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| Abstract: | Five main factors were screened by the Placket-Burman design method from ten ingredients of L-leucine basic fermentation medium. Then,the best level of five factors was determined by response surface analysis. The strain TQ9806-1 could produce L-leucine 18.05 g/L after fermentation with optimum medium for 72 hours by flask-shaking batch fermentation. |
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| Keywords: | L-leucine fermentation medium optimization response surface analysis |
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