| 杜仲细胞悬浮培养生产绿原酸的初步研究 |
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| 引用本文： | 王亚琴,叶青华,朱,媛.杜仲细胞悬浮培养生产绿原酸的初步研究[J].广西植物,2008,28(5):671-674. |
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| 作者姓名： | 王亚琴 叶青华 朱 媛 |
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| 作者单位： | 华南理工大学,生物科学与工程学院,广州,510640 |
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| 基金项目： | 广东省广州市科技计划，广东省国际科技合作计划 |
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| 摘 要： | 对影响杜仲细胞悬浮培养及其次生代谢物绿原酸产生的几种主要因子进行了研究。结果表明,在杜仲细胞悬浮培养生产绿原酸的过程中,第15天绿原酸的含量达到最大值。35g/L的蔗糖为最适碳源,MS培养基为最适悬浮培养基,pH为5.3时利于绿原酸的合成,2,4-D、NAA对绿原酸合成的促进效果不大,添加1.0mg/L的6-BA绿原酸的合成效果较好。
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| 关 键 词： | 杜仲 细胞悬浮培养 绿原酸 影响因子 |
Preliminary study on the cell suspension culture
of Eucommia ulmoides and secondary
metabolite-chlorogenic acid |
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| WANG Ya-Qin,YE Qing-Hu,ZHU Yuan.Preliminary study on the cell suspension culture
of Eucommia ulmoides and secondary
metabolite-chlorogenic acid[J].Guihaia,2008,28(5):671-674. |
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| Authors: | WANG Ya-Qin YE Qing-Hu ZHU Yuan |
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| Institution: | College of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510640, China |
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| Abstract: | Some major factors affected the cell suspension culture of Eucommia ulmoides and the production of chlorogenic acid were investigated. The results showed that the concentration of chlorogenic acid was the highest on 15th day,sucrose was better than glucose and maltose for cell growth and chlorogenic acid synthesis and the optimum concentration was 35 g/L. The optimal media was MS media and the proper pH value was 5.3. Some exogenous hormones such as 2,4-D and NAA had little effect on chlorogenic acid synthesis,but 1.0 mg/L 6-BA could promote the production of chlorogenic acid. |
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| Keywords: | Eucommia ulmoides cell suspension culture chlorogenic acid factors |
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