海石竹的离体快繁及核型分析

以海石竹 (Armeriamaritima)的叶片为外植体 ,经离体培养诱导产生愈伤组织 ,再分化形成不定芽 ,并经过继代增殖和壮苗生根 ,获得完整的再生植株 ,最后对其再生植株进行核型分析。结果表明 ,海石竹叶片的愈伤组织诱导和分化的适宜培养基为MS +BA 1 .0mg/L +NAA 0 .2mg/L ,诱导初期进行 7d暗培养 ,最佳增殖培养基为MS+BA 1 .0mg/L +NAA 0 .1mg/L ,生根培养基为MS+NAA 0 .2mg/L。以上培养基均含蔗糖3 0 g/L ,琼脂 5g/L ,pH 5 .8。海石竹的核型公式为 2n=2x=1 8=1 0m +8sm ,存在染色体数目变异的现象。

Propagation in vitro and karyotype analysis of Armeria maritima

The calli induction,redifferentiation to adventitious buds and propagation in vitro from leaves of thrift (Armeria maritima (Mill.)Willd) were described. After rooting the full plantlets were obtained. The regenerated plants were used for karyotype analysis. The optimal medium for calli induction and redifferentiation from leaves was Murashige and Skoog’s basal medium (MS),supplied with 1.0 mg/L 6-benzylaminopurine (BA),0.2 mg/L α-naphthalene acetic acid (NAA),30 g/L sugar and 5 g/L agar (pH 5.8). To culture firstly in dark for 7 days was better for inducing callus in the medium. Micropropagation medium was MS+BA 1.0 mg/L+NAA 0.1 mg/L. Rooting medium was MS+NAA 0.2 mg/L. All media above contained sucrose 30 g/L,agar 5 g/L,pH 5.8. Chromosome analysis of the regenerated plants showed that the karyotype formula of A. maritima was 2n=2x=18=10m+8sm. Aberrance of chromosome number was observed.