南美香瓜梨离体培养快速复壮繁殖的研究

南美香瓜梨茎段被移植在ＭＳ基本培养基上，其中添加有ＢＡ６．０（ｍｇ／Ｌ以下单位相同）、ＩＢＡ０．２培养４周后增殖到３．８倍。用其嫩叶切块，在含有２，４—Ｄ０．５，ＢＡ０．２５的ＭＳ培养基上先诱导形成绿色愈伤组织，然后转入含ＢＡ６．０和ＩＢＡ０．２的ＭＳ培养基上，产生丛生不定芽。将２．５～３．０ｃｍ的不定芽切下移入含ＩＢＡ０．５与０．２％活性炭，１／２ＭＳ无机盐的培养基上，２５天后诱导生根，移栽成活率达９０％以上。

STUDY ON THE RAPID PROPAGATION IN VITRO OF SOLANUM MURICATUM

The stem cuttings of Solanum muricatum Ait.were inoculated on MS base medium supplied with BA 6.0 mg/L(same unit in following supplemented mecdium),IBA 0.2. After 4 weeks of culture,the multiplication of buds were 3.8 times.The small cuttings of leaf tissue were induced to callus on the MS medium supplied with 2,4-D 0.5 and BA 0.25. Then many buds were induced on the callus by culturing on the MS medium supplied with BA 6.0 and IBA 0.2.The plantlets with many roots were grown on the 1/2 MS medium suppplied with IBA 0.5 and 0.2% active carbon, after 25 days of culture.The plantlets were transplanted into the soil and the rate of survivers were up to 90%.